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ProteomicsComparative analysis of label-free and 8-plex iTRAQ approach for quantitative tissue proteomic analysis
MS-based approaches have been successfully utilized for the comprehensive characterization of the cell proteome. However, in the case of quantitative proteomics the open question still remains, which quantification method is more suitable for identification of proteins with the altered abundance, pa...
ORGANISM(S): Homo sapiens (Human) 
2015-09-09 | PXD002170 | Pride
Lc-ms/ms Bladder cancer Tissue proteomics Label-free Itraq 
ProteomicsCardioprotection by selective SGLT2 inhibitors in a non-diabetic mouse model of myocardial ischemia/reperfusion injury: a class or a drug effect?
Major clinical trials with sodium glucose co-transporter-2 inhibitors (SGLT-2i) exhibit protective effects against heart failure events, whereas inconsistencies regarding the cardiovascular death outcomes are observed. Therefore, we aimed to compare the selective SGLT-2i empagliflozin (EMPA), dapagl...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2021-12-17 | MSV000088582 | MassIVE
ProteomicsLC-MS/MS based proteomics analysis of decellularized and non-decellularized mouse kidney tissue samples
Two C57Bl6/J male mice were sacrificed by injecting 200 microliters of the lethal anaesthetic Dolethal. Kidneys were perfused with Dulbeccos Phosphate Buffered Saline, dissected, decapsulated and weighted. Kidneys were decellularized using 3 freeze-thaw cycles (freezing at -80C, 30 minute long thawi...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-02-07 | MSV000094031 | MassIVE
ProteomicsShotgun analysis of nuclear proteins from mouse brain
Nuclei were isolated from the mouse brain. Proteins were extracted with addition of Bensonase and cleaned up by acetone precipitation. Proteis were resuspended in 50 mM ammonium bicarbonate and PPS Silent Surfactant. Samples were sonicated on ice to improve the solubility of proteins, then alkylated...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
MSV000079301 | MassIVE
ProteomicsHUVEC cell extracts for the investigation of collagen-induced cell signalling
We demonstrated that an abundant Collagen type I alpha 1-derived peptide inhibited collagen type I-induced endothelial cell migration in Human Umbilical Vein Endothelial Cells. We then applied mass spectrometry based proteomics to investigate the potential signalling pathways induced by collagen typ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-03-12 | MSV000097296 | MassIVE
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