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MetabolomicsUntargeted LC-MS metabolomics for the analysis of micro-scaled extracellular metabolites from hepatocytes
Metabolome analysis in micro physiological models is a challenge due to the low volume of cell culture medium (CCM). Here, we report a LC-MS-based untargeted metabolomics protocol for the detection of hepatocyte extracellular metabolites from micro-scale samples of CCM. Using a single LC-MS method w...
2021-01-04 | MTBLS1794 | MetaboLights
MetabolomicsAlignment of multiple metabolomics LC-MS datasets from disparate diseases to reveal fever-associated metabolites

Acute febrile illnesses are still a major cause of mortality and morbidity globally, particularly in low to middle income countries. The aim of this study was to determine any possible metabolic commonalities of patients infected with disparate pathogens that cause fever. Three liquid chromatogra...

2023-07-17 | MTBLS4895 | MetaboLights
MetabolomicsUntargeted LC-MS metabolomic profiling of Tulipa iliensis leaves during Botrytis tulipae infection
This study investigated the metabolic responses of Tulipa iliensis leaves to Botrytis tulipae infection using untargeted LC-MS metabolomics. Leaf tissues were collected at 0, 6, 12, 24, 48 and 72 h post-inoculation, with three biological replicates per time point. All samples were snap-frozen in liq...
2027-08-19 | MTBLS15393 | MetaboLights
MetabolomicsLC-MS-Based Serum Metabolomics Reveals Distinct Metabolic Signatures in Patients with Intracerebral Hemorrhage

Intracerebral hemorrhage (ICH) is a severe neurological disorder with high mortality and disability. Using LC-MS, we profiled serum metabolites from 20 patients with and without ICH, identifying 3,178 metabolites. ICH samples were enriched in benzene derivatives (15.63%), organic acids (12.47%), ...

2025-09-24 | MTBLS13030 | MetaboLights
MetabolomicsUntargeted LC-MS metabolomics of redclaw crayfish gonads
Untargeted LC-MS metabolomics was performed on 20 Cherax quadricarinatus gonad samples in four groups n=5 each plus three pooled QC samples. Positive- and negative-ion mzXML files were generated using a Thermo Scientific Orbitrap Exploris 120. The submitted files contain centroided full-scan MS1 dat...
2026-09-04 | MTBLS15185 | MetaboLights
MetabolomicsMetabolic Drug Response Phenotyping in Colorectal Cancer Organoids by LC-QTOF-MS
As metabolic rewiring is crucial for cancer cell proliferation, metabolic phenotyping of patient-derived organoids is desirable to identify drug-induced changes and trace metabolic vulnerabilities of tumor subtypes. We established a novel protocol for metabolomic and lipidomic profiling of colorecta...
2021-10-12 | MTBLS2130 | MetaboLights
MetabolomicsSteady state isotope tracing in C. elegans (LC-MS analyses)

The metabolic network, composed of thousands of metabolic reactions, is essential for any organism. Despite its importance, a systems level understanding of how metabolic network is wired by reactions that carry flux in unperturbed condition and rewired by transcriptionally activating or repressi...

2024-12-03 | MTBLS11741 | MetaboLights
MetabolomicsA Python-based pipeline for preprocessing LC-MS data for untargeted metabolomics workflows
Preprocessing data in a reproducible and robust way is one of the current challenges in untargeted metabolomics workflows. Data curation in liquid chromatography-mass spectrometry (LC-MS) involves the removal of unwanted features (retention time; m/z pairs) to retain only high-quality data for subse...
2020-11-21 | MTBLS1919 | MetaboLights
MetabolomicsOptimised LC-MS Metabolomic Profiling Reveals Emergent Changes to Monocyte Metabolism in Response to Lipopolysaccharide
Immunometabolism examines the links between immune cell function and metabolism. Dysregulation of immune cell metabolism is now an established feature of innate immune cell activation. Advances in liquid chromatography mass spectrometry (LC-MS) technologies have allowed discovery of unique insights ...
2025-11-17 | MTBLS6730 | MetaboLights
MetabolomicsFDR-controlled metabolite annotation for high-resolution imaging mass spectrometry imaging MS (LC-MS assay)
High-mass-resolution imaging mass spectrometry promises to localize hundreds of metabolites directly from tissues, cell cultures, and agar plates with cellular resolution, but is hampered by the lack of bioinformatics for automated metabolite identification. We developed the first bioinformatics fra...
2016-12-05 | MTBLS378 | MetaboLights
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