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UnknownThe E3 ubiquitin ligase XIAP restricts Anaplasma phagocytophilum colonization of Ixodes scapularis ticks.
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S-EPMC3814841 | biostudies-literature
ProteomicsMudPIT analysis of the polysome-associated proteins from Plasmodium falciparum
The Plasmodium falciparum strain 3D7 was cultured in human O+ erythrocytes at 5% haematocrit as previously described (Trager and Jensen, 1976). Cultures were synchronized twice at ring stage with 5% D-sorbitol treatments performed eight hours apart (Lambros and Vanderberg, 1979). Cultures (8% parasi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2016-03-29 | MSV000079613 | MassIVE
ProteomicsMudPIT analysis of the mRNA interactome from Plasmodium falciparum
The Plasmodium falciparum strain 3D7 was cultured in human O+ erythrocytes at 5% haematocrit as previously described (Trager and Jensen, 1976). Cultures were synchronized twice at ring stage with 5% D-sorbitol treatments performed eight hours apart (Lambros and Vanderberg, 1979). Cultures (8% parasi...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2016-03-29 | MSV000079612 | MassIVE
ProteomicsPfMORC protein regulates chromatin accessibility and transcriptional repression in the human malaria parasite, P. falciparum.
Enrichment of PfMORC-HA associated proteins by HA immunoprecipitation from P. falciparum parasites expressing P. falciparum MORC tagged with a 3xHA tag.
ORGANISM(S): Plasmodium Falciparum (ncbitaxon:5833) 
2023-07-06 | MSV000092353 | MassIVE
ProteomicsMudPIT analyses of the proteins associated with the CRWN-like protein isolated from mixed stages of Plasmodium falciparum intraerythrocytic development cycle
Protein pull-down assay- Mixed erythrocytic stage 3D7 Plasmodium falciparum parasite cultures were collected and lysed using 0.15% saponin for 10 min on ice. After subsequent washes, the parasite pellet was resuspended in 2.5X volume of IP buffer (0.65% Igepal CA-360 (Sigma-Aldrich), 50 mM Tris-HCl ...
ORGANISM(S): Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2019-12-10 | MSV000084671 | MassIVE
ProteomicsMudPIT analyses of the proteins associated with SMC3 isolated from mixed stages of Plasmodium falciparum intraerythrocytic development cycle
Protein pull-down assay- Mixed erythrocytic stage 3D7 Plasmodium falciparum parasite cultures were collected and lysed using 0.15% saponin for 10 min on ice. After subsequent washes, the parasite pellet was resuspended in 2.5X volume of IP buffer (0.65% Igepal CA-360 (Sigma-Aldrich), 50 mM Tris-HCl ...
ORGANISM(S): Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2018-06-28 | MSV000082521 | MassIVE
ProteomicsMudPIT analyses of the chromatin associated proteins isolated from the nucleus of Plasmodium falciparum at three time-points of the intraerythrocytic development cycle
Parasite cultures- Plasmodium falciparum strain 3D7 was cultured in human O+ erythrocytes at 5% hematocrit. Cultures were synchronized at ring stage with 5% (w/v) D-sorbitol treatments. Parasite cultures (8% parasitemia in 5% hematocrit) were harvested 48 hours after the first sorbitol treatment (ri...
ORGANISM(S): Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2018-06-28 | MSV000082520 | MassIVE
ProteomicsMudPIT analyses of the proteins co-purified with RAP01-HA, RAP21-HA, and negative controls from late asexual Plasmodium falciparum parasites
[NOTE: PF3D7_1470600 previously named RAP14 is now named RAP21]. HA-affinity purification - Purified late asexual parasites from parental and HA-tagged PF3D7_0105200 (RAP01-HA) and PF3D7_1470600 (RAP21-HA) lines were suspended in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM EDTA, 1 % Triton X-100, 1 mM ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2020-12-24 | MSV000086636 | MassIVE
ProteomicsHistone modifications in Plasmodium falciparum at 30 hours post-invasion
Synchronized Plasmodium falciparum-infected human blood cultures were harvested at seven time points along the asexual intra-erythrocytic parasite life cycle: 0 hours post-invasion, 6 hpi, 12 hpi, 18 hpi, 24 hpi, 30 hpi and 36 hpi. Plasmodium histones were extracted using the standard hydrochloric ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2015-08-12 | MSV000079230 | MassIVE
ProteomicsMultiple Reaction Monitoring assays targeting modified peptides from Plasmodium histones at 0, 18 and 36 hours post-invasion
Acid-extracted histones from the 0-hpi_2, 18-hpi_1 and 36-hpi_2 samples previously analyzed for PTMs by MudPIT were digested with endoproteinase Arg-C (RC) or endoproteinase Lys-C followed by Glu-C (KCEC). Digested histone peptides were separated on a 15cm nano-reverse phase (RP) column using a 4-hr...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2016-06-02 | MSV000079792 | MassIVE
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