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TranscriptomicsRNA-seq comparing allergen-trained versus untrained human monocyte-derived macrophages after HDM challenge
Mibrobial Patterns can induce trained innate immunity in macrophages. We tested whether innate memory can be elicited by stimulating macrophages with a house dust mite extract for 24h before washout and a resting phase of 5 days. Subsequently, macrophages were exposed to HDM for 24h and we analyzed ...
ORGANISM(S): Homo sapiens 
2021-12-31 | E-MTAB-9210 | biostudies-arrayexpress
TranscriptomicsRNA-seq comparing allergen-trained versus untrained human monocyte-derived macrophages
Mibrobial Patterns can induce trained innate immunity in macrophages. We tested whether innate memory can be elicited by stimulating macrophages with a house dust mite extract for 24h before washout, and analyzed the gene expression after 5 days post-exposure.
ORGANISM(S): Homo sapiens 
2021-12-31 | E-MTAB-9214 | biostudies-arrayexpress
TranscriptomicsRNA-seq of human monocyte-derived macrophages comparing healthy versus house dust mite-allergic donors
Human monocyte-derived macrophages (MDM) from HDM-allergic donors were compared to MDM from healthy donors. CD14+ monocytes were isolated from donated blood and differentiated in the presence of GM-CSF and TGFb (alveolar-like MDM) for 7 days before havest of RNA
ORGANISM(S): Homo sapiens 
2021-12-31 | E-MTAB-9215 | biostudies-arrayexpress
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