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We report the sites of protospacer integration into plasmids by Cas1-Cas2. The goal of this study is to determine the role of sequence elements on protospacer integration. Methods: Cas1-Cas2 integration products were fragmented, end-repaired, adapter ligated, PCR amplified, and analyzed by Illumina ...
ORGANISM(S): Cloning vector pUC19 
Both RNAi-dependent and -independent mechanisms have been implicated in the establishment of heterochromatin domains, which may be stabilized by feedback loops involving chromatin proteins and modifications of histones and DNA. Neurospora crassa sports features of heterochromatin found in higher eu...
ORGANISM(S): Neurospora crassa 
Biological production of aviation fuels has a realistic impact on global warming. Important sustainable aviation fuel (SAF) targets are emerging and isoprenol is recently identified as a precursor for a promising SAF compound DMCO (1,4-dimethylcyclooctane). Isoprenol has been produced in several eng...
ORGANISM(S): Pseudomonas Putida Kt2440 
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