Sort   by:  
 Page size 
TranscriptomicsIsl1-Lhx3 fusion specifies motor neuron fate by inducing motor neuron genes and concomitantly suppressing the interneuron programs
Combinatorial transcription codes generate the myriad of cell types during development, and thus likely provide crucial insights into directed differentiation of stem cells to a specific cell type. The LIM-complex composed of Isl1 and Lhx3 directs the specification of spinal motor neurons (MNs) in e...
ORGANISM(S): Mus musculus 
2012-02-03 | E-GEOD-35510 | biostudies-arrayexpress
GenomicsSTAT3 promotes motor neuron differentiation by collaborating with motor neuron-specific LIM complex
The motor neuron (MN)–hexamer complex consisting of LIM homeobox 3, Islet-1, and nuclear LIM interactor is a key determinant of motor neuron specification and differentiation. To gain insights into the transcriptional network in motor neuron development, we performed a genome-wide ChIP-sequencing an...
ORGANISM(S): Mus musculus 
2013-09-19 | E-GEOD-50993 | biostudies-arrayexpress
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from Saccharomyces cerevisiae by FLAG-affinity purification and Size Exclusion Chromatography
Yeast protein extractions and subcellular fractionation Yeast cells expressing FLAG tagged proteins were grown in YPD medium at 30oC to OD600 of 0.8. 12 Liters YPD culture of cells were pelleted and washed in SB buffer. The pellet was resuspended in SB buffer and treated with 2 mg/ml Zymolyase 20T ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-03-20 | MSV000085126 | MassIVE
ProteomicsMudPIT analyses of the pyruvate dehydrogenase complex (PDC) isolated from human
Mammalian protein extraction and Halo purification For Halo purification, HEK293T cells at 40% confluency in a 100 mm plate were transfected with 10ug of plasmid containing ORF of PDHA1-HA-Halo in 1X BBS with 125 mM CaCl2. Cells were harvested by scraping in PBS following PBS wash after 24 hours fro...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-03-20 | MSV000085125 | MassIVE
Sort   by:  
 Page size