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MetabolomicsThe role of FYCO1-dependent autophagy in lens fiber cell differentiation.

FYCO1 (FYVE and coiled-coil domain containing 1) is an adaptor protein, expressed ubiquitously and required for microtubule-dependent, plus-end-directed transport of macroautophagic/autophagic vesicles. We have previously shown that loss-of-function mutations in FYCO1 cause cataracts wit...

2024-03-24 | MTBLS206 | MetaboLights
MetabolomicsExamining the effects of cigarette smoke on mouse lens through a multi OMIC approach
Here, we report a multi OMIC (transcriptome, proteome, and metabolome) approach to investigate molecular changes in lens fiber cells (FC) of mice exposed to cigarette smoke (CS). Pregnant mice were placed in a whole-body smoke chamber and a few days later pups were born, which were exposed to CS for...
2024-03-24 | MTBLS207 | MetaboLights
MetabolomicsOvothiol A is the Main Antioxidant in Fish Lens
Tissue protection from oxidative stress by antioxidants is of vital importance for cellular metabolism. The lens mostly consists of fiber cells lacking nuclei and organelles, having minimal metabolic activity; therefore, the defense of the lens tissue from the oxidative stress strongly relies on met...
2019-07-30 | MTBLS806 | MetaboLights
TranscriptomicsDifferentiation state-specific mitochondrial dynamic regulatory networks are revealed by global transcriptional analysis of the developing chicken lens.
The mature eye lens contains a surface layer of epithelial cells called the lens epithelium that require a functional mitochondrial population to maintain the homeostasis and transparency of the entire lens. The lens epithelium overlies a core of terminally differentiated fiber cells that must degr...
ORGANISM(S): Gallus gallus 
2014-07-02 | E-GEOD-53976 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human lens epithelial cells and lens cortical fiber cells
Total RNA was isolated from three separate populations of human lens epithelial cells and three matching populations of lens cortical fiber cells. All samples were analyzed on separate microarrays.
ORGANISM(S): Homo sapiens 
2007-08-25 | E-GEOD-2256 | biostudies-arrayexpress
TranscriptomicsChromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation [lens tissue]
Genome-wide approach to identify the cell-autonomous role of Brg1 in lens fiber cell terminal differentiation. To examine roles of Brg1 in mouse lens development, a dnBrg1 transgenic construct was expressed using the lens-specific alphaA-crystallin promoter in postmitotic lens fiber cells. Morpholog...
ORGANISM(S): Mus musculus 
2011-01-01 | E-GEOD-22322 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of fiber cell maturation in the lens
The mammalian lens consists of an aged core of quiescent cells enveloped by a layers of mature fully elongated and younger, continuously elongating and transcriptionaly active cells. In the present study, we compared global gene expression profiles in elongating and mature, non-elongating fiber cell...
ORGANISM(S): Mus musculus 
2008-06-22 | E-GEOD-2083 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human lens epithelial cells to examine glucocorticoid induced changes
Prolonged use of glucocorticoids can lead to the formation of a cataract, however the mechanism is not known. We recently reported the presence of the functional glucocorticoid receptor in immortalized cultured mammalian lens epithelial cells (Gupta & Wagner, Invest Ophthalmol Vis Sci 2003), but the...
ORGANISM(S): Homo sapiens 
2007-09-01 | E-GEOD-3040 | biostudies-arrayexpress
ProteomicsTranscriptome, proteome, and metabolome of lens fiber cells from mice exposed to cigarette smoke
We adopted an omics (transcriptome, proteome, and metabolome) approach to characterize the lens fiber cells extracted from control (CT) and cigarette smoke (CS) exposed mouse (C57BL/6) eyes. The eight pregnant female mice (gestation days 19-20) were placed in a whole-body exposure smoking chamber an...
ORGANISM(S): Mus musculus (Mouse) 
2022-02-15 | PXD020421 | Pride
Mass-spectrometry Mouse lens fiber cells Tmt Proteome Cigarette smoke 
TranscriptomicsEctopic activation of Wnt/beta-catenin signaling in lens fiber cells results in cataract formation.
Transgenic mouse line called alphaA-CLEF in which transactivation domain of beta-catenin is fused to the amino terminus of DNA-binding protein LEF and the expression of transgenic protein is controlled by alphaA-crystallin promoter (-342/+49) was used to mimic the activation of canonical Wnt/beta-c...
ORGANISM(S): Mus musculus 
2014-02-11 | E-MTAB-1691 | biostudies-arrayexpress
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