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TranscriptomicsMulti-modal single-cell sequencing of differentiating mESCs using Smart-seq3 and scATAC-seq
We report dynamics of X-chromosome upregulation (XCU) along X-chromosome inactivation (XCI) in mESCs as they differentiate into EpiSCs. F1 hybrid C57BL6/J × CAST/EiJ male and female mESCs were grown in serum/LIF conditions were differentiated using Fgf2 and Activin A for 1, 2, 4 and 7 days to induce...
ORGANISM(S): Mus musculus 
2022-02-14 | E-MTAB-10714 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNA sequencing of differentiating mESCs using Smart-seq3
We report dynamics of X-chromosome upregulation (XCU) along X-chromosome inactivation (XCI) in mESCs as they differentiate into EpiSCs. F1 hybrid C57BL6/J × CAST/EiJ male and female mESCs were adapted to 2i/LIF and female cells grown in serum/LIF conditions were differentiated using Fgf2 and Activin...
ORGANISM(S): Mus musculus 
2022-02-14 | E-MTAB-9324 | biostudies-arrayexpress
TranscriptomicsIntroducing synthetic thermostable RNase inhibitors in single-cell RNA-seq (Bulk RNA-seq libraries)
Single-cell RNA-sequencing (scRNAseq) is revolutionizing biomedicine, propelled by advances in methodology, ease of use, and cost reduction of library preparation. Over the past decade, there have been remarkable technical improvements in most aspects of single-cell transcriptomics. Yet, there has b...
ORGANISM(S): Mus musculus 
2024-07-26 | E-MTAB-13899 | biostudies-arrayexpress
TranscriptomicsIntroducing synthetic thermostable RNase inhibitors in single-cell RNA-seq
Single-cell RNA-sequencing (scRNAseq) is revolutionizing biomedicine, propelled by advances in methodology, ease of use, and cost reduction of library preparation. Over the past decade, there have been remarkable technical improvements in most aspects of single-cell transcriptomics. Yet, there has b...
ORGANISM(S): Mus musculus 
2024-07-29 | E-MTAB-13873 | biostudies-arrayexpress
TranscriptomicsAllelic dilution series series of C57BL6/J:CAST/EiJ RNA using Smart-seq3
Technical control for allelic detection using Smart-seq3. Liver RNA from pure C57BL6/J and CAST/EiJ strains was combined at varying ratios (0:1, 1:7, 1:3, 3:5, 1:1, 5:3, 3:1, 7:1, 1:0) for a total of 200 pg RNA per sample.
ORGANISM(S): Mus musculus 
2022-02-14 | E-MTAB-10709 | biostudies-arrayexpress
TranscriptomicsGenome-wide 5-hydroxymethylcytosine (5hmC) profiling by hMeDIP-seq in primary human CD4+T-cells polarized towards Th1 and Th2 time-series
Differentiation of CD4+T-cells into effector subsets is a critical component of the adaptive immune system and an incorrect response can lead to autoimmunity or immune deficiency. Cellular differentiation including T-cell differentiation is accompanied by large-scale epigenetic remodeling, including...
ORGANISM(S): Homo sapiens 
2016-07-11 | E-MTAB-4686 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of primary human CD4+T-cells overexpressing TET1 polarized towards Th1
Differentiation of CD4+T-cells into effector subsets is a critical component of the adaptive immune system and an incorrect response can lead to autoimmunity or immune deficiency. Cellular differentiation including T-cell differentiation is accompanied by large-scale epigenetic remodeling, including...
ORGANISM(S): Homo sapiens 
2016-07-11 | E-MTAB-4685 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human CD4+T-cells, Th1/Th2 polarized time-series and primary memory subsets
Differentiation of CD4+T-cells into effector subsets is a critical component of the adaptive immune system and an incorrect response can lead to autoimmunity or immune deficiency. Cellular differentiation including T-cell differentiation is accompanied by large-scale epigenetic remodeling, including...
ORGANISM(S): Homo sapiens 
2016-07-11 | E-MTAB-4687 | biostudies-arrayexpress
TranscriptomicsDNA methylation profiling of primary human CD4+T-cell memory subsets
Differentiation of CD4+T-cells into effector subsets is a critical component of the adaptive immune system and an incorrect response can lead to autoimmunity or immune deficiency. Cellular differentiation including T-cell differentiation is accompanied by large-scale epigenetic remodeling, including...
ORGANISM(S): Homo sapiens 
2016-07-11 | E-MTAB-4689 | biostudies-arrayexpress
TranscriptomicsDNA methylation profiling of human CD4+T-cells in-vitro polarized towards Th1 and Th2 time-series
Differentiation of CD4+T-cells into effector subsets is a critical component of the adaptive immune system and an incorrect response can lead to autoimmunity or immune deficiency. Cellular differentiation including T-cell differentiation is accompanied by large-scale epigenetic remodeling, including...
ORGANISM(S): Homo sapiens 
2016-07-11 | E-MTAB-4688 | biostudies-arrayexpress
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