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ProteomicsIn-Search Assignment of Monoisotopic Peaks Improves the Identification of Cross-Linked Peptides
Cross-linking/mass spectrometry has undergone a maturation process akin to standard proteomics by adapting key methods such as false discovery rate control and quantification. A seldom-used search setting in proteomics is the consideration of multiple (lighter) alternative values for the monoisotopi...
ORGANISM(S): Homo sapiens (Human) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) 
2019-04-05 | PXD011121 | Pride
Data processing Cross-linking mass spectrometry 
ProteomicsReliable identification of protein-protein interactions by crosslinking mass spectrometry_CLMS_sSDA_datasets
Crosslink mass spectrometry datasets on sulfo-SDA-crosslinked affinity-purified RNA polymerase binders from SPA-pulldowns in E. coli K12, on rpoB-SPA / nusG-SPA / yacL-SPA. Cleared E. coli lysate was added to anti-FLAG M2 beads to isolate binders to rpoB (RNA polymerase), nusG or yacL (RNA polymer...
ORGANISM(S): Escherichia Coli 
2021-04-14 | PXD024148 | JPOST Repository
ProteomicsReliable identification of protein-protein interactions by crosslinking mass spectrometry_CLMS_BS3&DSSO_datasets
Crosslink mass spectrometry dataset on soluble, SEC-fractionated high-molecular weight proteome of E. coli, crosslinked with BS3 or DSSO. This data was used to identify protein-protein interactions and to obtain information on protein (complex) topologies. In the end, the data was used for finding ...
ORGANISM(S): Escherichia Coli 
2021-04-14 | PXD019120 | JPOST Repository
ProteomicsReliable identification of protein-protein interactions by crosslinking mass spectrometry_Protein coelution
Quantitative proteomics dataset on soluble, SEC-fractionated high-molecular weight proteome of E. coli for protein coelution analysis. This data was used to identify proteins for a subsequent crosslinking mass spectrometry search (DB creation) and to correlate protein elution behaviour to validate/...
ORGANISM(S): Escherichia Coli 
2021-04-14 | PXD019004 | JPOST Repository
ProteomicsReliable identification of protein-protein interactions by crosslinking mass spectrometry_AP-MS
Quantitative proteomics dataset on soluble, affinity-enriched proteins from SPA-pulldowns in E. coli K12, on rpoB-SPA / nusG-SPA / yacL-SPA. This data was used to identify proteins for enrichment analysis and subsequent crosslinking mass spectrometry search (DB creation). Cleared E. coli lysate was ...
ORGANISM(S): Escherichia Coli 
2021-04-14 | PXD024146 | JPOST Repository
ProteomicsProtein complexes in cells by AI-assisted structural proteomics (Proteomics)
Accurately modeling the structures of proteins and their complexes using artificial intelligence is revolutionizing molecular biology. Experimental data enable a candidate-based approach to systematically model novel protein assemblies. Here, we use a combination of in-cell crosslinking mass spectro...
ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168 
2023-02-16 | PXD035521 | JPOST Repository
ProteomicsProtein complexes in cells by AI-assisted structural proteomics (Cofractionation MS)
Accurately modeling the structures of proteins and their complexes using artificial intelligence is revolutionizing molecular biology. Experimental data enable a candidate-based approach to systematically model novel protein assemblies. Here, we use a combination of in-cell crosslinking mass spectro...
ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168 
2023-02-16 | PXD035520 | JPOST Repository
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