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UnknownValidation of a prognostic model and the impact of mutations in patients with lower-risk myelodysplastic syndromes.
Not available
S-EPMC3438234 | biostudies-literature
GenomicsA highly sensitive and robust method for genome-wide 5hmC profiling of rare cell populations
Investigations of 5-hydroxymethylcytosine (5hmC) in biologically and clinically samples and models with low cell numbers have been hampered by the low sensitivity and reproducibility using current 5hmC mapping approaches. Here, we develop a selective 5hmC chemical labeling approach using tagmentatio...
ORGANISM(S): Mus musculus 
2016-09-01 | E-GEOD-77967 | biostudies-arrayexpress
TranscriptomicsTet2-/-Flt3ITD and WT stem and progenitor cells
The study was a comparison of gene expression using RNA-seq. We analyzed the stem and progenitor cells from WT and Vav-cre+ Tet2fl/fl Flt3-ITD (T2F3) mice. We isolated stem cells LSK (lin- sca+ kit+) and granulocyte-macrophage progenitors GMP (lin- sca- kit+ fcgr+ cd34+) cells from bone marrow. Comp...
ORGANISM(S): Mus musculus 
2015-05-01 | E-GEOD-57244 | biostudies-arrayexpress
GenomicsASXL1 Mutations Promote Myeloid Transformation Through Loss of PRC2-Mediated Gene Repression
Recurrent somatic ASXL1 mutations occur in patients with myelodysplasia (MDS), myeloproliferative neoplasms (MPN), and acute myeloid leukemia (AML), and are associated with adverse outcome. Despite the genetic and clinical data implicating ASXL1 mutations in myeloid malignancies, the mechanisms of t...
ORGANISM(S): Homo sapiens 
2012-08-21 | E-GEOD-38861 | biostudies-arrayexpress
TranscriptomicsIntegrated genomic analysis illustrates the central role of JAK-STAT pathway activation in myeloproliferative neoplasm pathogenesis [HEL cell lines]
We used expression profiling, SNP arrays, and mutational profiling to investigate a well-characterized cohort of MPN patients. MPN patients with homozygous JAK2V617F mutations were characterized by a distinctive transcriptional profile. Notably, a transcriptional signature consistent with activated ...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-54645 | biostudies-arrayexpress
TranscriptomicsAnalysis of DNA methylation changes in normal human hematopoietic stem and progenitor cells
DNA methylation analysis on purified human long-term and short-term hematopoietic stem cells (LT-HSC, ST-HSC), common myeloid and megakaryocyte-erythrocyte progenitor cells (CMP, MEP) using HELP arrays. FACS-purified hematopoietic stem and progenitor cell (HSPC) subsets were analyzed for changes in ...
ORGANISM(S): Homo sapiens 
2014-02-07 | E-GEOD-52151 | biostudies-arrayexpress
TranscriptomicsIntegrated genomic analysis illustrates the central role of JAK-STAT pathway activation in myeloproliferative neoplasm pathogenesis [MPN patients]
We used expression profiling, SNP arrays, and mutational profiling to investigate a well-characterized cohort of MPN patients. MPN patients with homozygous JAK2V617F mutations were characterized by a distinctive transcriptional profile. Notably, a transcriptional signature consistent with activated ...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-GEOD-54644 | biostudies-arrayexpress
TranscriptomicsAnalysis of RNA expression changes in normal human hematopoietic stem and progenitor cells
RNA expression analysis on purified human long-term and short-term hematopoietic stem cells (LT-HSC, ST-HSC), common myeloid and megakaryocyte-erythrocyte progenitor cells (CMP, MEP) using microarrays. FACS-purified hematopoietic stem and progenitor cell (HSPC) subsets were analyzed for changes in R...
ORGANISM(S): Homo sapiens 
2014-02-07 | E-GEOD-52150 | biostudies-arrayexpress
TranscriptomicsExpression data from LSK WT, GMP WT and GMP NcstnKO
Notch signaling is one of the central regulators of differentiation in a variety of organisms and tissue types. Within the hematopoietic system, Notch is essential for the emergence of definitive HSC during fetal life and controls adult HSC differentiation to the T-cell lineage. Notch activation is ...
ORGANISM(S): Mus musculus 
2011-05-12 | E-GEOD-27811 | biostudies-arrayexpress
TranscriptomicsExpression data from LSK WT and LSK NcstnKO
Notch signaling is one of the central regulators of differentiation in a variety of organisms and tissue types. Within the hematopoietic system, Notch is essential for the emergence of definitive HSC during fetal life and controls adult HSC differentiation to the T-cell lineage. Notch activation is ...
ORGANISM(S): Mus musculus 
2011-05-12 | E-GEOD-27794 | biostudies-arrayexpress
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