LAPC4 cells were starved for 2 days and stimulated with 1µM 5α-Abi or 0.1nM DHT. Gene expression profiles are detected to determine the effect of 5a-Abi on prostate cancer cell line. LAPC4 cells were starved for 2 days with phenol red-free and serum free-medium and stimulated with 1µM 5α-Abi or...
Purpose: In order to understand the functional significance of sperm transcriptome in stallion fertility, the aim of this study was to generate a detailed body of knowledge about the sperm RNA profile that defines a normal fertile stallion. Methods: The 50 bp single-end ABI SOLiD raw reads were dire...
RNA isolation and purification steps greatly influence the results of gene expression profiling. There are two commercially available products for whole blood RNA collection, PAXgene and Tempus blood collection tubes, and each comes with their own RNA purification method. We examined the impact of R...
This experiment is contains a subset of mouse organism part samples and RNA-seq data from experiment E-GEOD-39524 (https://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-39524/), which was originally submitted by the ENCODE consortium to NCBI Gene Expression Omnibus under accession number GSE39524 (h...
To study the mechanism underlying IDA-induced anti-tumor activity for Abi resistance, LNCaP-Abi cells were treated with Abi alone and in combination with IDA for 24 h. Protein profiles were determined by mass spectrometry
Five hundred ng to 1 ug of genomic DNA was submitted to The Centre for Applied Genomics (TCAG) at The Hospital for Sick Children for genomic library preparation and whole genome sequencing. DNA samples were quantified using the Qubit High Sensitivity Assay and purity was assessed using the Nanodrop ...
To study the characteristics and mechanisms of Myc-induced zebrafish liver tumor, next-generation sequencing-based SAGE analyses were used to examine the transcriptomes of tumor and control samples. The results indicated that ribosome proteins were overwhelmingly up-regulated in the Myc-induced live...
