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Isobutanol has emerged as a potential biofuel due to recent metabolic engineering efforts. Here we used gene expression and transcription factor(TF)-gene interaction data, genetic knockouts, and Network Component Analysis (NCA) to map the isobutanol response network of Escherichia coli under aerobic...
ORGANISM(S): Escherichia coli 
The goal was to ascertain the impact of cyanide treatment on E. coli's transcriptome. There were 4 biological samples. For each sample there were two replicate DNA microarray performed. Data was analyzed with Imagene and lcDNA Hyduke DR, Rohlin L, Kao KC, Liao JC. 2003 A software package for cDNA ...
ORGANISM(S): Escherichia coli 
Th2 cells enable humoral immunity and host-defense to parasites. Whereas IL-4 drives Th2 differentiation and IL-2 is important later in this process by augmenting IL4 chromatin accessibility, we demonstrate that IL-2 serves an essential early role in regulating IL-4R? expression by inducing binding ...
ORGANISM(S): Mus musculus 
Purpose: The aim of this study is to identify genes that are under the transcriptional control of the epigenetic regulator Smchd1 in neural stem cells (NSCs) derived from E14.5 mouse brain Methods: Total RNA was extracted using an AllPrep DNA/RNA Mini Kit (Qiagen) from cultured neural stem cells der...
ORGANISM(S): Mus musculus 
RNA isolation and RNA-Seq: Wild-type Xcc or pXccBphP strains were cultured in far-red light or dark conditions up to logarithmic phase (0.7 to 0.8 OD600) at 28C in PYM broth. Total bacterial RNA was isolated using the MasterPureTM RNA Purification Kit (Epicentre, Illumina). Samples corresponding to ...
ORGANISM(S): Xanthomonas campestris pv. campestris 
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