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ProteomicsAUTO-SP: automated sample preparation for analyzing proteins and protein modifications
Liquid chromatography (LC) tandem mass spectrometry (MS/MS) is one of the widely used proteomic techniques to study the alterations occurred at protein level as well as post-translation modifications (PTMs) of proteins that are relevant to different physiological or pathological statuses. The mass s...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2026-03-16 | PXD064088 | Pride
Proteomics Automation Mass spectrometry Sample preparation Protein modifications 
ProteomicsSonication-assisted protein extraction improves proteomic detection of membrane-bound and DNA-binding proteins from tumor tissues
Tissue lysis using urea buffer was adopted in our previously published CPTAC protocol for mass spectrometry (MS)-based quantitative global proteomic and phosphoproteomic analysis. Herein, we reported an update to this initial protocol by implementing a sonication step into urea-based tissue lysis. S...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2025-07-28 | PXD050336 | Pride
Global proteomics Phosphoproteomics Tumor-associated proteomics Sonication 
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