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TranscriptomicsChromatin immunoprecipitation of mouse tumour cell line L1210 synchronized and sampled after mitosis to map replication time zones in the mouse genome
Measuring replication time of regions in teh mouse genome, using a novel synchronization method, labeling of BrdU, immunoprecipitation and hybridization to microarrays
ORGANISM(S): Mus musculus 
2008-08-04 | E-MEXP-1022 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse lymphoblast cell line L1210 to validate replication timing experiments
In this experiment, total RNA was extracted from asynchronous population of L1210 cells and hybridized to Affymetrix 430A 2.0 arrays in order to obtain an expression profile of these cells. We have previously mapped the replication timing of the entire mouse genome in this cell line, using mouse CGH...
ORGANISM(S): Mus musculus 
2008-08-04 | E-MEXP-1681 | biostudies-arrayexpress
TranscriptomicsProtocol Dependence of Sequencing-based Gene Expression Measurements
RNA samples from human tissues (brain and liver) and cell lines (K562 and HL60) were used to assess RNA fragmentation, RNA fractionation, cDNA synthesis, and single versus multiple tag counting. Technical replicates and different cDNA synthesis protocols were compared. Though protocols employing p...
ORGANISM(S): Homo sapiens 
2011-03-24 | E-GEOD-28123 | biostudies-arrayexpress
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