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12.5 ng of cfDNA was used as input for shallow whole-genome sequencing (sWGS), aiming for a coverage of x0.2-0.4-fold. Library preparation was performed using the TruSeq Nano DNA High Throughput Library Prep Kit (Illumina, San Diego, CA, USA) on an automated Hamilton STAR liquid handling system (Ham...
Eliminating B cells through anti-CD20 antibody led to a drastic reshaping of the inflammatory tumor microenvironment. This included a profound reduction of T cells, endothelial cells, monocytes and fibroblasts. The results of this study are therefore the first evidence in human that B cells are requ...
ORGANISM(S): Homo sapiens 
Purpose: To asses the transcriptome of 4 strains of the Tasmanian Devil facial tumor and one healthy fibroblast cell line. Methods: DFTD cells were grown from primary cell cultures derived from fine needle aspirates that have been collected from the wild. Total RNA from devil facial tumor strains 1-...
ORGANISM(S): Sarcophilus harrisii 
2019-01-14 | GSE108107 | GEO
This experiment showed a distinct phenotypic and functional change in B cells induced by melanoma conditioned medium. Therefore, B cells are directly activated by melanoma cells. It furthermore shows, that B cells express a wide variety of genes that indicate that B cells have a strong effect on the...
ORGANISM(S): Homo sapiens 
The impact of PDF supplementation with alanyl-glutamine (AlaGln) on peritoneal immune-competence in 6 patients in an open-label, randomized, crossover pilot trial (EudraCT 2012-004004-36) was tested by relating functional test results to transcriptome changes (RNAseq and miRNA analysis) in PD efflue...
ORGANISM(S): Homo sapiens 
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