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ProteomicsAfter enriching U251 cell lysate by a lactic probe with a photo-cross linker and a corresponding control probe without lactic modification to find related reader or eraser, the protein enriched were identified by Lc-MS (three replicates)
In brief, U251 cells lysate were incubated with 10μM KpKla and 10μM KpKla separately. After incubating for 1h at 37℃, the solutions were irradiated by 365nm UV for 5 min on ice. Then CuSO4, THPTA (tris-hydroxypropyltriazolylmethylamine), TCEP (tris(2-carboxyethyl)phosphine) and biotin azide were add...
ORGANISM(S): Homo Sapiens 
2023-01-29 | PXD039731 |
ProteomicsTargeted and non-targeted mass spectrometry to explore the chemical diversity of the genus Gambierdiscus in the Atlantic Ocean
The 15 strains of Gambierdiscus were cultivated in four separate laboratories. Each laboratory grew their available strains (see sample metadata) in addition to the G. australes strain AUS S080911_1 isolated from Pacific Ocean. Irradiance (70-100 umol photons m-2 s-1) and light/dark cycle (12h:12h)...
ORGANISM(S): Gambierdiscus Silvae (ncbitaxon:1550089) Gambierdiscus Carolinianus (ncbitaxon:864182) Gambierdiscus Australes (ncbitaxon:439317) Gambierdiscus Belizeanus (ncbitaxon:439316) Gambierdiscus Excentricus (ncbitaxon:986170) Gambierdiscus Caribaeus (ncbitaxon:864185) 
2024-02-26 | MSV000094172 | MassIVE
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