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ProteomicsRegulation of proteome by the proteasome upon ER stress
HeLa cells were with Tunicamycin or Thapsigargin for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-04-30 | MSV000083736 | MassIVE
ProteomicsProteomics analysis of HeLa cells starved with or without MG132
HeLa cells were amino acid starved for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin. The peptides...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-03-30 | MSV000083644 | MassIVE
ProteomicsComparison of Drosophila melanogaster adult and embryo proteome
Embryo and adult flies infected with Wolbachia pipientis or cured were lysed in a SDS containing buffer and digested with trypsin using in gel digestion. The resulting peptides were injected on a Sciex Triple-TOF 6600 in SWATH-MS mode. The data were analysed using Spectronaut 10 using the spectral l...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2018-08-10 | MSV000082812 | MassIVE
ProteomicsProteomics of HeLa cells starved and treated with YWF or leptomycin B
HeLa cells labeled with SILAC and were amino acids starved and treated with YWF, leptomycin B, MG132 or chloroquine. Cells were lysed with a 8M Urea buffer and proteins were reduced and alkylated. Equal amount of proteins of different labels were mixed and were digested with trypsin and resulting pe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-08-01 | MSV000092564 | MassIVE
ProteomicsDIA-PASEF–based FLAG-IP proteomic analysis of nuclear or cytosolic p62 condensates in HeLa cells
Cells were cross-linked with 0.1% formaldehyde for 10 min at 25 °C and quenched with 100 mM glycine. After washing with HBS (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl), cells were lysed in HEPES-RIPA buffer (20 mM HEPES-NaOH, pH 7.5, 150 mM NaCl, 1 mM MgCl₂, 1 mM EGTA, 1% NP-40, 0.25% sodium deoxycholat...
ORGANISM(S): Homo Sapiens (human) 
2025-12-30 | PXD072519 | JPOST Repository
ProteomicsDIA-PASEF–based total proteome profiling of HeLa cells expressing nuclear or cytosolic p62 condensates
Cells were lysed in 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 × g for 15 min at 4 °C. The supernatants were recovered, and proteins (20 µg each) were purified by the SP3 method an...
ORGANISM(S): Homo Sapiens (human) 
2025-12-30 | PXD072518 | JPOST Repository
ProteomicsRNF5 interactome
Cells expressing Flag-tagged RNF5 or empty vector were incubated with the proteasome inhibitor MG132 to block protein degradation. Cell lysates were then prepared and immunoprecipitationed with anti-Flag antibody were performed, and eluates were digested with trypsin and analyzed on a Q Exactive.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-25 | MSV000083160 | MassIVE
ProteomicsSPANX immunoprecipitation analysis by mass spectrometry
A375 cells transduced with empty pLVX or pLVX-SPANXA-FLAG were subjected to immunoprecipitation using Flag beads. Elution from beads was performed by incubation with 3XFlag peptide for 1h at 4 C and eluates were subjected to tryptic digestion followed by LC-MS/MS, as described previously (PMID: 3099...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-24 | MSV000084617 | MassIVE
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