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ProteomicsLC-MS/MS analysis of immunoprecipitation samples of Tetrahymena meiotic proteins Melg1, Melg2, Melg3, and Tass1
In most eukaryotes, the meiotic chromosomal bouquet (comprising clustered chromosome ends) provides an ordered chromosome arrangement that facilitates pairing and recombination between homologous chromosomes. In the protist Tetrahymena thermophila, the meiotic prophase nucleus stretches enormously a...
ORGANISM(S): Tetrahymena thermophila 
2020-11-23 | PXD021813 | Pride
Tetrahymena thermophila Meiosis Immunoprecipitation Lc-ms/ms 
ProteomicsLC-MS/MS analysis of immunoprecipitation samples of Tetrahymena Mediator complex associated proteins
In the germline nucleus of Tetrahymena and other ciliates there occurs a peculiar burst of transcriptional activity during sexual reproduction. A major part of the genome is transcribed into noncoding RNA (ncRNA). This ncRNA serves to spot transposable elements (TEs) and other unwanted DNA sequences...
ORGANISM(S): Tetrahymena thermophila SB210 
2019-07-11 | PXD012372 | Pride
Tetrahymena thermophila Emit1 Emit2 Mediator complex Rib1 
ProteomicsLC-MS/MS analysis of immunoprecipitation samples of Tetrahymena meiotic proteins Mcmd1 and Pamd1
Recombinational repair of meiotic DNA double-strand breaks (DSBs) uses the homologous chromosome as a template, although the sister chromatid offers itself as a spatially more convenient substrate. In many organisms, this choice of the homolog rather than the sister is reinforced by the recombinatio...
ORGANISM(S): Tetrahymena thermophila 
2019-11-19 | PXD015986 | Pride
Tetrahymena thermophila Meiosis Immunoprecipitation Lc-ms/ms 
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