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ProteomicsMudPIT analyses of the proteins associated with the CRWN-like protein isolated from mixed stages of Plasmodium falciparum intraerythrocytic development cycle
Protein pull-down assay- Mixed erythrocytic stage 3D7 Plasmodium falciparum parasite cultures were collected and lysed using 0.15% saponin for 10 min on ice. After subsequent washes, the parasite pellet was resuspended in 2.5X volume of IP buffer (0.65% Igepal CA-360 (Sigma-Aldrich), 50 mM Tris-HCl ...
ORGANISM(S): Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2019-12-10 | MSV000084671 | MassIVE
ProteomicsMudPIT analyses of the proteins associated with SMC3 isolated from mixed stages of Plasmodium falciparum intraerythrocytic development cycle
Protein pull-down assay- Mixed erythrocytic stage 3D7 Plasmodium falciparum parasite cultures were collected and lysed using 0.15% saponin for 10 min on ice. After subsequent washes, the parasite pellet was resuspended in 2.5X volume of IP buffer (0.65% Igepal CA-360 (Sigma-Aldrich), 50 mM Tris-HCl ...
ORGANISM(S): Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2018-06-28 | MSV000082521 | MassIVE
ProteomicsMudPIT analyses of the chromatin associated proteins isolated from the nucleus of Plasmodium falciparum at three time-points of the intraerythrocytic development cycle
Parasite cultures- Plasmodium falciparum strain 3D7 was cultured in human O+ erythrocytes at 5% hematocrit. Cultures were synchronized at ring stage with 5% (w/v) D-sorbitol treatments. Parasite cultures (8% parasitemia in 5% hematocrit) were harvested 48 hours after the first sorbitol treatment (ri...
ORGANISM(S): Plasmodium Falciparum 3d7 (ncbitaxon:36329) 
2018-06-28 | MSV000082520 | MassIVE
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