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TranscriptomicsTesting the limits of 454 pyrotag sequencing: reproducibility, quantitative assessment and comparison to T-RFLP fingerprinting of aquifer microbes
The characterization of microbial community structure via 16S rRNA gene profiling has been greatly advanced in recent years by the application of amplicon pyrosequencing. The possibility of barcode-tagged sequencing of templates gives the opportunity to massively screen multiple samples from environ...
ORGANISM(S): Aliivibrio fischeri 
2012-07-16 | E-GEOD-35631 | biostudies-arrayexpress
TranscriptomicsElectron acceptor-dependent identification of key anaerobic toluene degraders at a tar-oil contaminated aquifer by Pyro-SIP
Bioavailability of electron acceptors is probably the most limiting factor in the restoration of anoxic, contaminated environments. The oxidation of contaminants such as aromatic hydrocarbons, particularly in aquifers, often depends on the reduction of ferric iron or sulphate. We have previously det...
ORGANISM(S): unidentified 
2011-03-18 | E-GEOD-25449 | biostudies-arrayexpress
TranscriptomicsHigh-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment
Contaminated aquifer (Dusseldorf-Flinger, Germany) templates extracted from 5 sediment depths ranging between 6.4 and 8.4 m below ground and over 3 years of sampling were amplified for amplicon pyrosequencing using the primers Ba27f (5’-aga gtt tga tcm tgg ctc ag-3’) and Ba519r (5’- tat tac cgc ggc ...
ORGANISM(S): unidentified 
2012-11-11 | E-GEOD-25345 | biostudies-arrayexpress
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