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ProteomicsMass spectrometric profiling of lysine malonylation in CRISPRi 562 cells lines to investigate malonylation activity of lysine acetyltransferases
Lysine malonylation is a protein posttranslational modification with potentially broad biological impact. We present a protocol to generate stable gene knockdown K562 cell lines through lentiviral infection of a CRISPRi system followed by lysine malonylation measurement using mass spectrometry (MS)....
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-12-04 | MSV000093564 | MassIVE
ProteomicsSIRT5 Regulates Lysine Malonylation
Protein acylation links energetic substrate flux with cellular adaptive responses. SIRT5 is a NAD+-dependent lysine deacylase and removes both succinyl and malonyl group. Using affinity enrichment and label free quantitative proteomics, we characterized the SIRT5-regulated lysine malonylome in wild ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2015-04-27 | MSV000079116 | MassIVE
ProteomicsLysine crotonylation, 2-hydroxyisobutyrylation, acetylation, and malonylation profiles in OSCC.
Liquid chromatography-tandem mass spectrometry (LC‒MS/MS) analysis was performed for lysine crotonylation (KCr), 2-hydroxyisobutyrylation (Khib), acetylation (Kac), and malonylation (Kma) to obtain a comprehensive profile and reveal the differences in modified proteins between OSCC and adjacent tiss...
ORGANISM(S): Homo sapiens (Human) 
2026-09-21 | PXD073784 | Pride
Oral squamous cell carcinoma  lysine 2-hydroxyisobutyrylation  lysine crotonylation  lysine acetylation  lysine malonylation 
ProteomicsFirst Global Proteomic Analysis of Lysine Malonylation in Toxoplasma gondii
Lysine malonylation (Kmal) is a new posttranslational modification (PTM), which has been reported in several prokaryotic and eukaryotic species. Although Kmal can regulate many and diverse biological processes in various organisms, knowledge about this important PTM in the apicomplexan parasite Toxo...
ORGANISM(S): Toxoplasma gondii 
2020-05-27 | PXD015809 | Pride
Post-translational  modifications Toxoplasma gondii Malonylome Lysine malonylation 
ProteomicsNOVEL ROLE OF FATTY ACID SYNTHASE IN ANGIOGENESIS THROUGH MODULATION OF LYSINE MALONYLATION OF MTOR
The role of fatty acid synthesis (FAS) in endothelial cells (ECs) remains incompletely characterized. In this project, we found that silencing of fatty acid synthase (FASN) impedes vessel sprouting by reducing EC proliferation. Moreover, loss of FASN in ECs impaired angiogenesis in vivo, and FASN bl...
ORGANISM(S): Homo sapiens (Human) 
2019-07-08 | PXD006503 | Pride
Protein malonylation Fatty acid synthesis Endothelial cell metabolism 
ProteomicsDiurnal regulation of Acyl-CoA synthetase 3 (ACSF3) governs rhythmic mitochondrial lysine-malonylation and daily hepatic metabolism
Hepatic daily rhythms are coordinated by feeding and the molecular circadian clock, ensuring metabolic homeostasis. Disrupted feeding schedules promote circadian misalignment and metabolic disease but the underlying mechanisms remain unknown. Post-translational modifications have emerged as key regu...
ORGANISM(S): Mus musculus (Mouse) 
2026-07-07 | PXD066863 | Pride
Quantitative proteomics Acsf3 Mouse Circadian rhythms Acyl-coa synthetase 3 Silac Liver Lysine-malonylation 
ProteomicsQuantitative Analysis of Lysine Malonylation Proteome Unravels Physiological and Biochemical Mechanism in Responding to Salicylic Acid in Roots of Platycodon grandiflorus
Salicylic acid, as a plant hormone, significantly affects physiological and biochemical indexes of such as soluble sugar, malondialdehyde content, peroxidase and superoxide dismutase enzyme activity in Platycodon grandiflorus. Lysine malonylation is a post-translational modification that involves va...
ORGANISM(S): Platycodon grandiflorus 
2025-05-07 | PXD058676 | Pride
Platycodon grandiflorus  salicylic acid  lysine malonylation  medicinal plants  qualitative proteome  post-translational modification. 
ProteomicsMalonylation of Acetyl-CoA Carboxylase 1 Promotes Hepatic Steatosis and Is Attenuated by Ketogenic Diet in NAFLD
Protein post-translational modifications (PTMs) participate in important bioactive regulatory processes and therefore can help elucidate the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Here, we investigate the involvement of PTMs in ketogenic diet (KD)-improved fatty liver by multi-om...
ORGANISM(S): Mus musculus (Mouse) 
2023-07-21 | PXD040481 | Pride
Lysine malonylation  hepatic steatosis  acetyl-coa carboxylase 1  ketogenic diet. 
GenomicsDiurnal regulation of Acyl-CoA synthetase 3 (ACSF3) governs rhythmic mitochondrial lysine-malonylation and daily hepatic metabolism
Diurnal regulation of Acyl-CoA synthetase 3 (ACSF3) governs rhythmic mitochondrial lysine-malonylation and daily hepatic metabolism
PRJNA1463862 | ENA
ProteomicsComparative Analysis Reveals Genotype-specific Lyisne Malonylation of Toxoplasma gondii
Toxoplasma gondii is a protozoan parasite which can infect a wide range of animals, including humans. According to virulence, it is generally divided into three different strains, namely type I (RH strain), type II (PRU strain) and type III (VEG strain). Lysine malonylation (Kmal) is a new type of p...
ORGANISM(S): Toxoplasma gondii VEG 
2022-05-11 | PXD029366 | Pride
Toxoplasma gondii Differentially expressed proteins (deps) Lysine malonylation Genotype 
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