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Common cell lysis procedures distort ribosome profiling analyses of gene expression
We evaluated different in-solution and FASP-based sample preparation strategies for absolute protein quantification. Label-free quantification (LFQ) was employed to compare different sample preparation strategies in the bacterium Pseudomonas aeruginosa and human embryonic kidney cells (HEK) and note...
ORGANISM(S): Homo sapiens (Human) Pseudomonas aeruginosa PAO1 
2015-10-06 | PXD001511 | Pride
Hypoxia in the tumor microenvironment plays a crucial role in the evasion of immune surveillance by tumor cells. In this study, we aimed to identify the miRs regulated by hypoxia in NSCLC and melanoma and their putative involvement in the regulation of tumor susceptibility to antigen-specific cytoto...
ORGANISM(S): Homo sapiens 
The experiment was designed to obtain a broader unbiased view of the changes in islet macrophages following low dose STZ challenge. Mice were purchased from Jackson Laboratory (Bar Harbor, ME). 16-20-week-old C57BL/6J males were given 30 mg/kg STZ or acetate buffer (control) i.p. (intraperitoneal ...
ORGANISM(S): Mus musculus 
The coiled-coil domain of EPG5 was synthesized as follows: Biotin-MNKNEAVSQQIHVLQKEVRQLQAE AAQ (from Beijing SciLight Biotechnology, LCC). 1 mg of this short peptide was incubated with ES cells lysates followed by immunoprecipitation with anti-biotin beads. The precipitates were extensively washed w...
ORGANISM(S): Mus musculus (Mouse) 
2019-02-25 | PXD012563 | Pride
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