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MetabolomicsShotgun lipidomics of HeLa whole-cell lysates and lysosomes following LLOMe treatment

Perturbations in lysosome integrity are tightly linked to neurological disorders and ageing, but the underlying pathogenic mechanisms are incompletely understood. Using an unbiased proteomic approach, we here identified the bridge-like lipid transport protein VPS13C/PARK23 as a key component of a...

2026-07-03 | MTBLS14737 | MetaboLights
MetabolomicsCholesterol maintains the degradative capacity of lysosomes during clearance and recycling of dysfunctional mitochondria
Efficient clearance and recycling of dysfunctional mitochondria through the robust catabolic activity of lysosomes is essential for cellular health. However, how membrane lipids contribute to maintaining the degradative capacity of lysosomes remains poorly understood. Here, we show that cholesterol ...
2026-07-30 | MTBLS15181 | MetaboLights
TranscriptomicsAMPK governs lineage specification through Tfeb-dependent regulation of lysosomes
Faithful execution of developmental programs relies on the acquisition of unique cell identities from pluripotent progenitors, a process governed by combinatorial inputs from numerous signaling cascades that ultimately dictate lineage-specific transcriptional outputs. Despite growing evidence that m...
ORGANISM(S): Mus musculus 
2016-03-09 | E-GEOD-77705 | biostudies-arrayexpress
GenomicsPeripheral positioning of lysosomes supports melanoma aggressiveness
Peripheral positioning of lysosomes supports melanoma aggressiveness
PRJEB64554 | ENA
ProteomicsTwo step enrichment facilitates background reduction for proteomic analysis of lysosomes
Lysosomes comprise the main degradative compartment of almost all mammalian cells and are involved in various cellular functions, most of which are catalyzed by the lysosomal proteome. Lysosomal proteins are low abundant, complicating their analysis by mass spectrometry-based proteomics. To increase...
ORGANISM(S): Homo sapiens (Human) 
2024-08-01 | PXD048696 | Pride
Lysosomes  lysosome enrichment  superparamagnetic iron oxide particles (spions)  tmem192 Mass spectrometry  proteomics  organelles Hek 293 
ProteomicsMouse osteoclast secretory lysosomes nLC-ESI-MS/MS
To survey the proteome of osteoclast secretory lysosomes, we used superparamagnetic iron oxide nanoparticles (SPIONs) to enrich for these endo-lysosomal-related organelles from murine osteoclast cultures. Briefly, large scale murine bone marrow monocyte (BMM)-derived osteoclast cultures were ‘pulsed...
ORGANISM(S): Mus musculus (Mouse) 
2023-01-25 | PXD037006 | Pride
Lysosome Lro Mouse Spion Osteoclast Secretory lysosome 
GenomicsLysosomes mediate the mitochondrial UPR via mTORC1-dependent ATF4 phosphorylation
Lysosomes mediate the mitochondrial UPR via mTORC1-dependent ATF4 phosphorylation
PRJNA744091 | ENA
ProteomicsComprehensive proteome analysis reveals the central role of macrophage lysosomes in regulating immune responses
Phagocytosis and autophagy in macrophages have been shown to be essential to both innate and adaptive immunity. Lysosomes are the main catabolic subcellular organelles responsible for degradation and recycling of both extracellular and intracellular material, which are the final steps in phagocytosi...
ORGANISM(S): Mus musculus (Mouse) 
2017-04-24 | PXD002915 | Pride
Innate immunity Proteomics Macrophage lysosome Pathogen 
TranscriptomicsPeripheral positioning of lysosomes supports melanoma aggressiveness
Emerging evidences suggest that both function and position of organelles are pivotal for tumor cell dissemination. Among them, lysosomes stand out as they integrate metabolic sensing with gene regulation and secretion of proteases. Yet, how lysosomes function is linked to their position and thereby ...
ORGANISM(S): Homo sapiens 
2023-07-31 | E-MTAB-13165 | biostudies-arrayexpress
ProteomicsProteins enriched on damaged lysosomes in 293T
Lysosomal membrane permeabilization (LMP) or lysosomal membrane damage is commonly associated with aging and age-related diseases. In searching for cellular mechanisms in response to LMP, we used a proteomic approach to identify proteins enriched on damaged lysosomes. This unbiased approach identifi...
ORGANISM(S): Homo sapiens (Human) 
2022-09-07 | PXD028852 | Pride
Human Lysosomal membrane permeabilization 
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