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TranscriptomicsHCT 116 cells expressing Fucci (fluorescent ubiquitination-based cell cycle indicator); G1 phase vs. S/G2/M phase
Fucci-expressing HCT116 were inoculated into subcutaneous tissue or cecum of NOD/SCID mice. Four weeks after inoculation, Fucci green (mAG; S/G2/M) and red (mKO2;G1) expressing cells were isolated from implanted tumors by FACSAria sorting (BD, Biosciences). Comparative analyses among green and red c...
ORGANISM(S): Homo sapiens 
2014-01-09 | E-GEOD-34940 | biostudies-arrayexpress
TranscriptomicsThe G1 arrest and maintenance mechanism between the long-term G1-arrested and the G1-arrested cells.
Non mitotic tumor cells are resistant to conventional chemotherapeutic drugs. However, the mechanisms underlying this phenomenon remain unclear. Here, we found a population that is viable but remains in the G1 phase for an extended period of time (up to 48 h) by Long-term time-lapse observations in ...
ORGANISM(S): Homo sapiens 
2015-01-27 | E-GEOD-61088 | biostudies-arrayexpress
ProteomicsMature proteins of LAG-3 with or without mutations were digested with chymotrypsin followed by data-dependent LC-MS/MS analysis
The FLAG-tagged soluble monomeric proteins of LAG-3-WT, LAG-3-V20A, and LAG-3-L14Q/V20A were immunoprecipitated from culture supernatants of Plat-E cells transfected with the corresponding plasmid vectors using ANTI-FLAG M2 Magnetic Beads. Proteins on the beads were digested with chymotrypsin (Roche...
ORGANISM(S): Homo Sapiens (human) 
2022-04-11 | PXD023929 | JPOST Repository
ProteomicsN-terminal peptides of LAG-3 (WT, V20A, and L14Q/V20A) were quantified by PRM
The abundance of N-terminal fragments starting from V21 (pV21–W33), S22 (pS22–W33), S23 (pS23–W33), and G24 (pG24–W33) along with three control peptides (Ctrl1, pH81–Y95; Ctrl2, pR106–L118; Ctrl3, pH113–L123) were measured by parallel reaction monitoring (PRM), an MS/MS-based targeted quantification...
ORGANISM(S): Homo Sapiens (human) 
2022-04-11 | PXD023930 | JPOST Repository
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