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Drosophila miR-277 is down-regulated during adult life. To infer the transcripts regulated by miR-277, we transgenically expressed the miRNA from a heterologous promotor. RNA was extracted at an age when 50% of the population had died, then we compared the transgenic strain with constitutive express...
ORGANISM(S): Drosophila melanogaster 
Drosophila melanogaster Schneider cells (S2) were transfected with a 2'-O-methyl inhibitor antisense to miR-277 or an unrelated control sequence. Three days after inhibition of the miRNA, the cellular RNA was pulse-labeled with 4-thio-Uridine for 1 hour, then tthe cells were lysed and RNA was extrac...
ORGANISM(S): Drosophila melanogaster 
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