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ProteomicsLC-MS/MS data from spider major ampullate silk fibers and glands
This project contains proteomic (LC-MS/MS) data from 27 samples. The samples are as follows: (1) Major ampullate glands dissolved in 8M Urea (3 replicates); (2) Major ampullate silk fibers dissolved in 8M Urea (3 replicates); (3) Major ampullate silk fibers dissolved in Hexafluoroisopropanol (3 re...
ORGANISM(S): Larinioides sclopetarius 
2024-07-16 | PXD052116 | Pride
Major ampullate silk Major ampullate glands Spider silk Lc-ms/ms 
ProteomicsWestern black widow major ampullate silk gland and silk fiber LC-MSMS, Part 1
Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous comparison of tissue-specific RNAseq l...
ORGANISM(S): Latrodectus hesperus (Western black widow spider) 
2015-08-25 | PXD002592 | Pride
Major ampullate silk fibers Spidroins Spider Latrodectus hesperus Draglines Major ampullate silk glands 
ProteomicsWestern black widow major ampullate, minor ampullate, and tubuliform silk gland LC-MSMS, Part 2
Spider silk research has largely focused on spidroins, proteins that are the primary components of spider silk fibers. Although a number of spidroins have been characterized, other types of proteins associated with silk synthesis are virtually unknown. Previous comparison of tissue-specific RNAseq l...
ORGANISM(S): Latrodectus hesperus (Western black widow spider) 
2015-08-25 | PXD002595 | Pride
Minor ampullate silk glands Spidroins Spider Tubuliform silk glands Latrodectus hesperus Major ampullate silk glands 
ProteomicsMS characterization of spidroin-1
For MS characterization of major ampullate spidroin-1 from the three species of Nephila clavipes, edulis and madagascariensis, an experimental approach was developed combining 2-DE with multiple proteolytic in-gel digestion (trypsin, chymotrypsin, pepsin, proteinase K, subtilisin and proteinase 1...
ORGANISM(S): Nephila clavipes (Golden silk orbweaver) 
2022-02-22 | PXD000057 | Pride
Major ampullate silk Nephila sp. Post-translational modifications Phosphorylation Nanolc-esi-cid/etd-ms/ms Spidroin-1 
UnknownStructural Diversity of Native Major Ampullate, Minor Ampullate, Cylindriform, and Flagelliform Silk Proteins in Solution.
Not available
S-EPMC7421538 | biostudies-literature
ProteomicsComposition of minor ampullate silk makes its properties different from those of major ampullate silk
Spider’s minor ampullate silk, or MI-silk, exhibits distinct mechanical properties and water resistance compared to its major ampullate counterpart (MA-silk). The principal protein constituent of MI-silk is known as minor ampullate spidroin, or MiSp, and while its sequence has been deciphered and is...
ORGANISM(S): Cellular Organisms 
2023-12-18 | PXD038879 | JPOST Repository
UnknownExtraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin.
Not available
S-EPMC6206087 | biostudies-literature
UnknownMeta-analysis reveals materiomic relationships in major ampullate silk across the spider phylogeny.
Not available
S-EPMC7536055 | biostudies-literature
UnknownAnti-Coagulant and Antimicrobial Recombinant Heparin-Binding Major Ampullate Spidroin 2 (MaSp2) Silk Protein.
Not available
S-EPMC8869596 | biostudies-literature
UnknownIdentification and characterization of multiple Spidroin 1 genes encoding major ampullate silk proteins in Nephila clavipes.
Not available
S-EPMC2831225 | biostudies-literature
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