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TranscriptomicsIdentifying reference genes with stable expression from high throughput sequence data
Genes that are constitutively expressed across multiple environmental stimuli are crucial to quantifying differentially expressed genes, particularly when employing quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) assays. However, the identification of these potential reference...
ORGANISM(S): Thalassiosira pseudonana CCMP1335 
2013-01-01 | E-GEOD-40509 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse isolated granulocyte macrophage progenitors were incubated with a retrovirus that expressed either GFP or MLL-AF9 and GFP
Leukemias and other cancers possess a rare population of cells capable of self-renewal, and eradication of these cancer stem cells is likely necessary for long-term cancer-free survival. Given that both normal and cancer stem cells are capable of self-renewal the extent to which cancer stem cells re...
ORGANISM(S): Mus musculus 
2008-06-13 | E-GEOD-4416 | biostudies-arrayexpress
GenomicsEpigenetic profiling of hematopoietic stem cells and leukemia stem cells
The histone 3 lysine 79 (H3K79) methyltransferase Dot1l has been implicated in the development of leukemias bearing translocations that involve the Mixed Lineage Leukemia (MLL) gene. We identified the MLL-fusion targets in a murine MLL-AF9 leukemia model, and conducted epigenetic profiling for H3K7...
ORGANISM(S): Mus musculus 
2011-09-13 | E-GEOD-29130 | biostudies-arrayexpress
TranscriptomicsThe important role of GPR84 in MLL leukemogenesis
Beta-catenin signaling is required for establishment of leukemic stem cells (LSCs) in acute myeloid leukemia (AML), yet the upstream regulators that can augment this pathway are unknown. Through genome-wide gene expression analysis and functional studies, we identified an important role for GPR84 in...
ORGANISM(S): Mus musculus 
2014-10-15 | E-MTAB-1932 | biostudies-arrayexpress
TranscriptomicsMsi2 sustains the MLL leukemia stem cell regulatory program
Leukemia stem cells (LSCs) are found in most aggressive myeloid diseases and contribute to therapeutic resistance. Genetic and epigenetic alterations cause a dysregulated developmental program in leukemia. The MSI2 RNA binding protein has been previously shown to predict poor survival in leukemia. W...
ORGANISM(S): Mus musculus 
2015-01-15 | E-GEOD-64545 | biostudies-arrayexpress
GenomicsIncreased DNA Methylation of Dnmt3b-Targets Impairs Leukemogenesis (RRBS)
The de novo DNA methyltransferases Dnmt3a and Dnmt3b are of crucial importance in hematopoietic stem cells, and Dnmt3b has recently been shown to play a role in genic methylation. Forced Dnmt3b expression induced widespread DNA hypermethylation in myc-bcl2 induced leukemias, especially at promoters ...
ORGANISM(S): Mus musculus 
2016-01-11 | E-GEOD-71039 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human hematopoietic stem/progenitor cells treated with TGF-beta1 to idetntify its target genes (reference series)
Leukemias and other cancers possess a rare population of cells capable of self-renewal, and eradication of these cancer stem cells is likely necessary for long-term cancer-free survival. Given that both normal and cancer stem cells are capable of self-renewal the extent to which cancer stem cells re...
ORGANISM(S): Mus musculus 
2008-06-12 | E-GEOD-3725 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of the response of Arabidopsis cultivar Columbia etiolated seedlings and undifferentiated tissue culture cells to the spaceflight environment
We address a key baseline question of whether gene expression changes are induced by the orbital environment, and then we ask whether undifferentiated cells, cells presumably lacking the typical gravity response mechanisms, perceive spaceflight. Arabidopsis seedlings and undifferentiated cultured Ar...
ORGANISM(S): Arabidopsis thaliana 
2012-03-08 | E-MTAB-1009 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse bone marrow cells with a conditional Mef2c knockout or a Mef2c rescue
Acute myelogenous leukemia (AML) is driven by leukemic stem cells (LSC) generated by mutations that confer (or maintain) self-renewal potential coupled to an aberrant differentiation program. Using retroviral mutagenesis, we identified genes that generate LSC in collaboration with genetic disruption...
ORGANISM(S): Mus musculus 
2009-08-15 | E-GEOD-17231 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human cord blood cells transduced with Leukemia fusion-gene
MLL-AF9 expression in normal human umbilical cord blood CD34+ cells leads to long-term proliferation of a myeloid progenitor cell with leukemogenic potential. Expression of a Core Binding Factor leukemia fusion (AML1-ETO or CBFbeta-SMMHC) in human CD34+ cells results in self-renewal of primitive pro...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7011 | biostudies-arrayexpress
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