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TranscriptomicsThe NIH Human Pluripotent Stem Cell Database (Agilent, miRNA)
MicroRNA profiling was performed on RNA samples matched to those included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Twenty undifferentiated human embryonic stem cell lines and 4 human tissues were analyzed. Expanded descriptions of methods used are available at: http://stemc...
ORGANISM(S): Homo sapiens 
2012-10-31 | E-GEOD-34199 | biostudies-arrayexpress
TranscriptomicsThe NIH Human Pluripotent Stem Cell Database (Agilent, mRNA)
To better understand the extent to which induced pluripotent stem cells (iPSCs) faithfully recapitulate the characteristics of embryonic stem cells (ESCs) under (undiff)erentiated condition, KSR condition and FBS condition and how both compare to somatic tissues under these conditions, we employed w...
ORGANISM(S): Homo sapiens 
2012-10-31 | E-GEOD-32923 | biostudies-arrayexpress
TranscriptomicsThe NIH Human Pluripotent Stem Cell Database (Illumina, snp)
Genotyping was performed on DNA samples matched to the RNA samples included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Seventeen undifferentiated human embryonic stem cell lines and 11 human induced pluripotent stem cells were analyzed. Expanded descriptions of methods used a...
ORGANISM(S): Homo sapiens 
2012-10-31 | E-GEOD-35157 | biostudies-arrayexpress
TranscriptomicsThe NIH Human Pluripotent Stem Cell Database (Illumina, methylation)
Methylation profiling was performed on DNA samples matched to the RNA samples included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Nineteen undifferentiated human embryonic stem cell lines and 5 human induced pluripotent stem cells were analyzed. Expanded descriptions of metho...
ORGANISM(S): Homo sapiens 
2012-10-31 | E-GEOD-34869 | biostudies-arrayexpress
TranscriptomicsThe NIH Human Pluripotent Stem Cell Database (Agilent, cgh)
Array comparative genomic hybridization was performed on DNA samples matched to the RNA samples included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Twenty two undifferentiated human embryonic stem cell lines were analyzed. Expanded descriptions of methods used are available a...
ORGANISM(S): Homo sapiens 
2012-10-31 | E-GEOD-35735 | biostudies-arrayexpress
TranscriptomicsThe NIH Human Pluripotent Stem Cell Database (Affymetrix, mRNA)
To broaden the appeal of the NIH Stem Cell Database, we analyzed a subset of undifferentiated human embryonic stem cell lines (5 lines in duplicate) on the Affymetrix platform. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used a...
ORGANISM(S): Homo sapiens 
2012-10-31 | E-GEOD-33789 | biostudies-arrayexpress
TranscriptomicsHeparin promotes cardiac differentiation of human pluripotent stem cells in chemically defined albumin-free medium enabling consistent manufacture of cardiomyocytes
Cardiomyocytes can be differentiated from human pluripotent stem cells (hPSCs) in defined conditions, but efficient and consistent cardiomyocyte differentiation often requires expensive reagents such as B27 supplement or recombinant albumin. Using a chemically defined albumin-free (E8 basal) medium,...
ORGANISM(S): Homo sapiens 
2016-07-28 | E-GEOD-81711 | biostudies-arrayexpress
TranscriptomicsComparison of the molecular profiles of human embryonic and isogenic induced pluripotent stem cells. [Illumina]
Many studies have compared the genetic and epigenetic profiles of human induced pluripotent stem cells (hiPSCs) to human embryonic stem cells (hESCs) and yet the picture remains unclear. To address this, we derived a population of neural precursor cells (NPCs) from the H1 (WA01) hESC line and genera...
ORGANISM(S): Homo sapiens 
2014-01-01 | E-GEOD-51747 | biostudies-arrayexpress
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