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TranscriptomicsAn epigenetic role for maternally inherited piRNAs in transposon silencing
In plants and mammals, small RNAs indirectly mediate epigenetic inheritance by specifying cytosine methylation. Here, we find that small RNAs themselves serve as vectors for epigenetic information. Crosses between Drosophila strains that differ in the presence of a particular transposon can pr...
ORGANISM(S): Drosophila melanogaster 
2008-11-28 | E-GEOD-13081 | biostudies-arrayexpress
TranscriptomicsVreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila.
Here, we analyzed small RNA libraries derived from ovarian tissues heterozygous or mutant for the Tudor gene, Vreteno. In the absence of vret, Piwi-bound piRNAs are lost without changes in piRNA precursor transcript production, supporting a role for Vret in primary piRNA biogenesis. In the germline,...
ORGANISM(S): Drosophila melanogaster 
2011-08-15 | E-GEOD-30088 | biostudies-arrayexpress
TranscriptomicsThe exon junction complex controls transposable element activity by ensuring faithful splicing of the piwi transcript
The exon junction complex (EJC) is a highly conserved ribonucleoprotein complex which binds RNAs during splicing and remains associated with them following export to the cytoplasm. While the role of this complex in mRNA localization, translation and degradation has been well characterized, its mecha...
ORGANISM(S): Drosophila melanogaster 
2014-08-14 | E-GEOD-59327 | biostudies-arrayexpress
TranscriptomicsA rapid and scalable system for studying gene function in mice using conditional RNA interference
Four libraries were analyzed to assess the level and effects of inducing the ectopic expression of a luciferase targeting miRNA in mouse embryonic fibroblasts (MEFs). Here, we find low level, leaky expression in the absence of ROSA-rtTA and doxycyclin. When expression is effectively induced, the Luc...
ORGANISM(S): Mus musculus 
2010-12-01 | E-GEOD-19476 | biostudies-arrayexpress
TranscriptomicsVreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila
In Drosophila, Piwi proteins associate with Piwi-interacting RNAs (piRNAs) and protect the germline genome by silencing mobile genetic elements. This defense system acts in germline and gonadal somatic tissue to preserve germline development. Genetic control for these silencing pathways varies great...
ORGANISM(S): Drosophila melanogaster 
2011-09-28 | E-GEOD-30360 | biostudies-arrayexpress
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