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MetabolomicsNew plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediat...
2022-03-18 | MTBLS670 | MetaboLights
TranscriptomicsTranscription profiling of human pancreatic duct epithelial cell line (H6c7) Gene expression changes induced by K-ras oncogene in the
k-ras oncogene was transduced into the HPV16-E6E7 immortalized normal human pancreatic duct epithelial cell line (H6c7) to generate a stable K-ras oncogene-expressing cell line (H6c7-Kr). The gene expression profile of the H6c7-Kr cells was compared to that of H6c7 cells. We have excluded (subtracte...
ORGANISM(S): Homo sapiens 
2008-06-11 | E-GEOD-1955 | biostudies-arrayexpress
TranscriptomicsA gene expression signature associated with K-Ras addiction reveals regulators of EMT and tumor cell survival
K-Ras mutations occur frequently in epithelial cancers. Using shRNAs to deplete K-Ras in lung and pancreatic cancer cell lines harboring K-Ras mutations, two classes were identified—lines that do or do not require K-Ras to maintain viability. Comparing these two classes of cancer cells revealed a g...
ORGANISM(S): Homo sapiens 
2009-03-06 | E-GEOD-15126 | biostudies-arrayexpress
TranscriptomicsGene expression profiles of spontaneous metastasis in a K-ras/p53 mutant mouse model
The biologic basis for NSCLC metastasis is not well understood. Here we addressed this deficiency by transcriptionally profiling tumors from a genetic mouse model of human lung adenocarcinoma that develops metastatic disease owing to the expression of K-rasG12D and p53R172H. We identified 2,209 gene...
ORGANISM(S): Mus musculus 
2009-04-14 | E-GEOD-14449 | biostudies-arrayexpress
TranscriptomicsMutant K-ras promotes carcinogen-induced murine colorectal tumourigenesis, but does not alter tumour chromosome stability
K-ras mutations are observed in around 40% human colorectal adenomas and carcinomas and contribute to the pathogenesis of human and rodent colorectal tumour formation. Previously, we developed and characterised a strain of transgenic mice with inducible intestinal epithelial expression of K-rasVal12...
ORGANISM(S): Mus musculus 
2010-10-02 | E-GEOD-24010 | biostudies-arrayexpress
TranscriptomicsTranscription profile of K-ras activated murine menstruating uteri
The expression of K-ras transgene is induced in K-rasV12+/-/Cre+/+/ROSA26R-LacZ+/+ transgenic mice uteri, which were primed with hormone treatment. Decidualization was artificially induced in these uteri and hormone injection was withdrawn to induced menstruation-like endometrial degeneration. Micr...
ORGANISM(S): Mus musculus 
2010-06-20 | E-GEOD-15518 | biostudies-arrayexpress
MetabolomicsIdentification of BolA protein reveals a novel virulence factor in Klebsiella pneumoniae that contributes to liver abscess in mice

BolA has been characterized as an important transcriptional regulator, which is induced in the stationary phase of growth and is often associated with bacterial virulence. This study was initiated to elucidate the role of the BolA in the virulence of K. pneumoniae. Using a mous...

2022-09-13 | MTBLS2712 | MetaboLights
TranscriptomicsChromatin immunoprecipitation of human erythroleukemia cell line K-562 with H3K4me2, H3K4me3, H3ac, H4ac, Histone H2B and Histone H3 antibodies using an ENCODE array
ENCODE ChIP-chip study using human myelogenous leukemia cell line K-562 and anti histone H3K4me2 (Abcam; ab7766); H3K4me3 (Abcam; ab8580); H3ac (Upstate; 06-599); H4ac (Upstate; 06-866); Histone H2B (Abcam: ab1790) and Histone H3 (Abcam: ab1791) antibodies. Each antibody experiment was conducted in ...
ORGANISM(S): Homo sapiens 
2006-09-01 | E-TABM-140 | biostudies-arrayexpress
TranscriptomicsCathepsin K Contributes to Cavitation and Collagen Turnover in Pulmonary Tuberculosis
Cavitation in tuberculosis (TB) enables highly efficient person-to-person aerosol transmission. Currently, a number of hypotheses exist regarding the mechanisms underlying this process. To explore this process, we undertook an unbiased next-generation transcriptional analysis of samples from cavity ...
ORGANISM(S): Oryctolagus cuniculus 
2015-11-03 | E-GEOD-68206 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human HCMV-Infected monocytes
Human cytomegalovirus induces a pro-inflammatory monocyte following infection and we have evidence that NF-κB and phosphatidylinositol 3-kinase [PI(3)K] are key mediators in this early activation. To begin to address how these signalling pathways are responsible for the rapid activation of infecte...
ORGANISM(S): Homo sapiens 
2008-06-16 | E-GEOD-9601 | biostudies-arrayexpress
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