Yeast protein extractions and subcellular fractionation
Yeast cells expressing FLAG tagged proteins were grown in YPD medium at 30oC to OD600 of 0.8. 12 Liters YPD culture of cells were pelleted and washed in SB buffer. The pellet was resuspended in SB buffer and treated with 2 mg/ml Zymolyase 20T ...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)