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The aim was to carry out global analysis of gene expression changes occurring in the normal pubertal mouse mammary gland from the appearance to the regression of terminal end buds. Experiment Overall Design: 5 time points were chosen for analysis: 3wk, 4wk, 5wk, 6wk and 7wk. Each time point was anal...
ORGANISM(S): Mus musculus 
TGF-beta1 is the major cytokine driver of fibrotic scarring observed in diabetic nephropathy and other fibrosis-related diseases. RNA-sequencing offers the potential for more sensitive assessment of the TGF-M-CM-^_1-driven transcriptome. There were two treatment groups: vehicle, 48 hr TGFb1. Each tr...
ORGANISM(S): Homo sapiens 
RNA-seq was performed for transcriptional analysis of MCF10A cells, an epithelial mammary cell line. MCF10A cells were cultured in 3D acinus forming conditions (in Matrigel). Timepoints analysed were 24h, 34h, 36h, 38h and 48h into acinus formation. Control cells were monoloayer.
ORGANISM(S): Homo sapiens 
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