Massively parallel reporter assay

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Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay

We apply a massively parallel reporter assay (MPRA) that relies on mRNA and plasmid tag sequencing (Tag-Seq) to compare the regulatory activities of more than 27,000 distinct variants of two inducible enhancers in human cells: a synthetic cAMP-regulated enhancer and the virus-inducible interferon be...

ORGANISM(S): Escherichia coli

2012-02-26 | E-GEOD-31982 | biostudies-arrayexpress

Systematic dissection of regulatory motifs in 2,000 predicted human enhancers using a massively parallel reporter assay

We employ a massively parallel reporter assay (MPRA) to measure the ex vivo activities of hundreds of K562 and HepG2 enhancers with known transcription factor motif instances. For seven selected motifs that correspond to known or predicted activators and repressors in the two cell types, we make dir...

ORGANISM(S): Escherichia coli

2013-03-15 | E-GEOD-33367 | biostudies-arrayexpress

Massively parallel splicing reporter assay on designed sequence libraries

PRJNA545827 | ENA

A massively parallel reporter assay for RNA localization in neurons

PRJNA723817 | ENA

Characterization of maturational enhancer activity by massively parallel reporter assay [amplicon-Seq]

PRJNA804494 | ENA

Massively parallel reporter assay for programmed ribosomal frameshifting on designed sequence libraries

PRJNA607955 | ENA

Massively parallel reporter assay for identification of RNA switches

PRJNA1105614 | ENA