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TranscriptomicsTranscription profiling of human adenocarcinoma cell line A549 over-expressing ELMOD2
ELMOD2 cDNA from the IMAGE clone 3897166 was cloned into the pDsRed-Monomer-N1 expression vector. ELMOD2 was overexpressed in the A549(adenomcarcinoma)cell line and cells transfected with empty vector were considered controls. Transcript profiles were compared with the Affymetrix Hgu133plus2 arrays....
ORGANISM(S): Homo sapiens 
2009-12-07 | E-MEXP-1838 | biostudies-arrayexpress
TranscriptomicsTranscrioptome analysis of human primary macrophages stimulated by bacterial endotoxin lipopolysaccharide (LPS), and (1,3)-b-glucans, curdlan and glucan from bakerM-BM-4s yeast (GBY)
We performed gene expression microarray experiments to compare the global transcriptional response induced by b-glucans and LPS with their secretomes. We identified 1683, 767 and 1447 genes with over two-fold increase or decrease in LPS-, curdlan- or GBY-stimulated macrophages, respectively. We show...
ORGANISM(S): Homo sapiens 
2014-05-15 | E-GEOD-32282 | biostudies-arrayexpress
ProteomicsProteomic and bioinformatic characterization of extracellular vesicles released from human primary macrophages upon influenza A infection
We have previously shown that IAV infection of human monocyte-derived macrophages causes major alterations to subcellular protein trafficking inside the cell and triggers robust protein secretion (Lietzén et al, 2011). Here, we have used high-throughput proteomics to identify proteins secreted in ex...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-16 | MSV000080643 | MassIVE
ProteomicsProteomic and bioinformatic characterization of proteins secreted conventionally and unconventionally from human primary macrophages upon LPS transfection
The non-canonical caspase-4/5 inflammasome has been only very recently characterized. It is expressed by macrophages, the principal effector cells of the innate immunity system, and is activated by gram-negative bacteria, ER stress, and UV radiation. Activation of this inflammasome results in pyropt...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080769 | MassIVE
ProteomicsPhosphoproteomic characterization of infulenza A virus infected human macrophages
Human primary macrophages were infected with influenza A virus (H3N2/Udorn strain, HA 256) for 6 hrs or left untreated. Cells were collected and lysed with HEPES lysis buffer (50 mM HEPES, 150 mM NaCl, 1 mM EDTA, 1 % NP-40, pH 7.4) including protease and phosphatase inhibitor cocktails. The cell lys...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080717 | MassIVE
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