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Recombinant Cyanidioschyzon Dnm1 protein (CmDnm1) was incubated with recombinant Cyanidioschyzon Aurora kinase (CmAUR). Proteins were tryptic digested and the phosphorylated peptides were concentrated with Titansphere Phos -TiO Kit. Finally, the enriched peptides were analyzed by LC-MS.
ORGANISM(S): Cyanidioschyzon Merolae 
Recombinant Cyanidioschyzon Dnm1 protein (CmDnm1) was incubated with or without recombinant Cyanidioschyzon Aurora kinase (CmAUR). Proteins were separated by SDS-PAGE, in-gel tryptic digested, analyzed by LC-MS.
ORGANISM(S): Cellular Organisms 
Proteomic analysis revealed potential phospho-acceptor sites within Vpx by PIM3. HEK293 cells were transfected with plasmid vector encoding Vpx together with either empty vector or PIM3. Immunoprecipitated Vpx was subjected to liquid chromatography tandem-mass spectrometry.
ORGANISM(S): Homo Sapiens (human) 
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