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TranscriptomicsExpression profiles of a panel of MSS and MSI colon cancer cell lines
Expression profiling was used to identify genes differentially expressed in MSS (microsatellite stable) and MSI (microsatellite unstable) colon cancer cell lines. Data submitted in support of manuscript entitled Villin expression is frequently lost in poorly differentiated colon cancer, Diego Arang...
ORGANISM(S): Homo sapiens 
2012-02-07 | E-GEOD-35566 | biostudies-arrayexpress
ProteomicsEngineered live bacteria suppress Pseudomonas aeruginosa lung infection in mouse and dissolve biofilm in endotracheal tubes from patients
Engineered live bacteria could provide a new modality for treating lung infections , a major cause of mortality worldwide. Here, we engineered a genome-reduced human lung bacterium, Mycoplasma pneumoniae, to treat ventilator-associated pneumonia (VAP), a disease with high hospital mortality when ass...
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
2023-10-24 | PXD037233 | Pride
Engineered live bacteria  pseudomonas aeruginosa  lung infection  mouse  biofilm  endotracheal tubes 
GenomicsTransposon random mutagenesis of Mycoplasma pneumoniae M129 using ProTInSeq protocol along different concentrations and using two positive selection markers (Chloramphenicol acetyltransferase and Erythromycin esterase EreA) and a negative selection marker (barnase)
Definition of different Tn4001-derived mini-transposons carrying reporters with no translation initiation codons so they can only be expressed when fused to an endogenous protein. We used Chloramphenicol acetyltransferase (Cm) as a positive selection marker while the RNAse barnase (Barn) was used as...
ORGANISM(S): Mycoplasma pneumoniae M129 
2023-07-31 | E-MTAB-10380 | biostudies-arrayexpress
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