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ProteomicsMudPIT analyses of the proteins co-purified with the junction-localized tumor suppressors Scribble (Scrib) and Discs Large (Dlg) by immunoprecipitation from fly embryos
Fly embryos (w1118, Dlg-GFP, or Scrib-GFP) were lysed with lysis buffer (25mM Tris-HCl, 150mM EDTA, 1% NP-40, 5% glycerol, 1mM DTT, 1x protease inhibitor). Debris was removed by centrifugation, and extracts were cleared by incubation with Dynabeads (1 hour). Immunocomplexes were formed by incubation...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
MSV000082907 | MassIVE
ProteomicsSkeletal Muscle Metabolism of the Astyanax mexicanus
The muscle composition of cave dwelling and surface fish was analyzed by proteomics. Specifically, the composition of the myosin heavy chain was determined by in-gel digestion followed by reversed phase LC-MS/MS. The proteome of the different muscle types was analyzed by tandem mass tags and phosph...
ORGANISM(S): Astyanax Mexicanus (ncbitaxon:7994) 
2021-02-12 | MSV000086857 | MassIVE
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