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TranscriptomicsmiR-522 Pulldown-seq in MDA-MB-468
Identifying miRNA-regulated genes is key to understanding miRNA function. However, many miRNA recognition elements (MREs) do not follow canonical seed base-pairing rules, making identification of bona fide targets challenging. Here, we apply an unbiased sequencing-based systems approach to character...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-MTAB-2112 | biostudies-arrayexpress
TranscriptomicsmiR-522 IMPACT-seq in MDA-MB-468
Identifying miRNA-regulated genes is key to understanding miRNA function. However, many miRNA recognition elements (MREs) do not follow canonical seed base-pairing rules, making identification of bona fide targets challenging. Here, we apply an unbiased sequencing-based systems approach to character...
ORGANISM(S): Homo sapiens 
2014-06-03 | E-MTAB-2119 | biostudies-arrayexpress
TranscriptomicsProbing the off-target effect of EGFP siRNA and pro-siRNA in the HeLa-d1EGFP cell line
We have develped a novel method of making siRNAs (named pro-siRNA for prokaryotic siRNA). To evaluate off-targeting of pro-siRNA, we compared the mRNA expression profiles of HeLa-d1EGFP cells transfected with 4 nM EGFP siRNAs and pro-siRNAs by microarray. We used microarray to study the off-target e...
ORGANISM(S): Homo sapiens 
2013-02-12 | E-GEOD-44256 | biostudies-arrayexpress
TranscriptomicsmiR-522 Overexpression in MDA-MB-468
Over-expression of miR-522 mimic or control miR mimic in MDA-MB-468 breast cancer cell line
ORGANISM(S): Homo sapiens 
2014-06-30 | E-MTAB-2110 | biostudies-arrayexpress
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