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The aim of this study was to investigate transcriptomic changes in Clostridioides difficile when cultured as monoculture biofilms or as co-culture biofilms with either Bacteroides (Phocaeicola) dorei or Bacteroides (Phocaeicola) vulgatus. This study was also interested in transcriptomic changes occu...
ORGANISM(S): Phocaeicola vulgatus 
RNA-seq was conducted to uncover the regulatory roles of RsbW in the lifestyle of Clostridioides difficile strain R20291. RNA were extracted from planktonic cultures, which were grown in BHI-S (Sigma-Aldrich, USA) to early stationary phase (10h). The cells were re-suspended in LETS buffer and lysed...
ORGANISM(S): Clostridioides difficile 
Metabolomics offers new insights into disease mechanisms that is enhanced when adopting orthogonal instrumental platforms to expand metabolome coverage, while also reducing false discoveries by independent replication. Herein, we report the first inter-method comparison when using multisegment injec...
2023-08-22 | MTBLS3111 | MetaboLights
This experiment was performed largely to capture the dynamic transcriptional changes occurring in C. difficile when cultured within a 9 species synthetic gut communal consortium over a 48 h time series when cultured as a biofilm in vitro.
ORGANISM(S): Clostridioides difficile 
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