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TranscriptomicsAdipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity (differentiation data)
We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity. Subcutaneous adipose tissue was obtained from healthy subjects undergoing co...
ORGANISM(S): Homo sapiens 
2012-08-12 | E-GEOD-25910 | biostudies-arrayexpress
TranscriptomicsAdipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [miRNA data]
We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity. Abdominal subcutaneous adipose needle biopsies were obtained from women (n=5...
ORGANISM(S): Homo sapiens 
2012-08-12 | E-GEOD-25470 | biostudies-arrayexpress
TranscriptomicsAdipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity [gene expression]
We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity. Abdominal subcutaneous adipose needle biopsies were obtained from women (n=5...
ORGANISM(S): Homo sapiens 
2012-08-12 | E-GEOD-25401 | biostudies-arrayexpress
GenomicsEarly B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue [ChIP-seq]
Examination of EBf1 binding by ChIP-seq in differentiated human adipose stromal cell (hASC) pre-adipocyte Pre-Adipocytes differentiated in-vitro were fixed in 1% formaldehyde for 15 min at room temperature and quenched for 5 min by adding glycine to a final concentration of 0.125 M. ChIP assays wer...
ORGANISM(S): Homo sapiens 
2014-05-22 | E-GEOD-54889 | biostudies-arrayexpress
TranscriptomicsEarly B-cell Factor 1 Regulates Adipocyte Morphology and Lipolysis in White Adipose Tissue [expression profiling]
To investgate the role of EBF1 in human adipocyte, we performed global expression profiling in human adipocytes transfected with siRNA targeting EBF1. Human adipocytes differentiated in vitro were transfected with siRNA targeting EBF1 or Non-targeting Control. The cells were incubated for approximat...
ORGANISM(S): Homo sapiens 
2014-05-22 | E-GEOD-42680 | biostudies-arrayexpress
TranscriptomicsPRRX1 transcription factor association to type 2 diabetes
Primary human adipose stromal cells (hASCs) from rs4684847 CC risk allele carriers were cultured and induced to differentiate into adipocytes, and simultaneously transfected for 72h with non-targeting siRNA or siRNA targeting PRRX1 (n=10) or both PRRX1 and PPARG (subset of the subjects, n=4).
ORGANISM(S): Homo sapiens 
2013-12-01 | E-MTAB-1906 | biostudies-arrayexpress
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