Sort   by:  
 Page size 
ProteomicsOne-Pot Time-Induced Proteome Integral Solubility Alteration (OPTI-PISA) assay for automated and sensitive drug target identification
Proteome Integral Solubility Alteration (PISA) assay is widely used for identifying drug targets, but it is labour-, sample and time-consuming. Aiming at enabling automation and greatly reducing the sample amount, we developed One-Pot Time-Induced (OPTI)-PISA. Here we demonstrate OPTI-PISA performan...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD050241 | Pride
Drug target Proteome integral solubility alteration (pisa) One pot Automation 
ProteomicsGel-Assisted Proteome Position Integral Shift (GAPPIS) Assay Returns Molecular Weight to Shotgun Proteomics and Identifies Novel Caspase 3 Substrates
Here we present a high-throughput virtual top-down proteomics approach that restores the molecular weight (MW) information in shotgun proteomics, and demonstrate its utility in studying proteolytic events in programmed cell death. With Gel-Assisted Proteome Position Integral Shift (GAPPIS), we quant...
ORGANISM(S): Homo sapiens (Human) 
2025-03-11 | PXD049007 | Pride
Molecular weight，shotgun proteomics，mass spectrometry Caspase 3 Gel electrophoresis 
Sort   by:  
 Page size