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TranscriptomicsGeneration of NPM1-ALK translocation in human T lymphocytes faithfully recapitulates the diversity of ALK+ ALCL tumor phenotype
To recapitulate the t(2;5)(p23;q35) chromosomal translocation, activated T lymphocytes from healthy donors PBMC were transfected with the RNP complex composed of the protein Cas9 with gRNA NPM1 (targeting NPM1 gene) and gRNA ALK (targeting ALK gene). Control cells were wild-type CD4+ activated lymp...
ORGANISM(S): Homo sapiens 
2022-02-09 | E-MTAB-10924 | biostudies-arrayexpress
TranscriptomicsIdentification of ETO2-GLIS2 transcriptional targets
Acute megakaryoblastic leukemia (AMKL) is a subtype of leukemia primarily diagnosed in childhood and generally associated with poor prognosis. Genetic alterations found in de novo childhood AMKL include the OTT-MAL fusion, MLL and NUP98 fusions and the recently identified ETO2-GLIS2 fusion that invo...
ORGANISM(S): Homo sapiens 
2017-05-05 | E-MTAB-4332 | biostudies-arrayexpress
TranscriptomicsIdentification of Acute megakaryoblastic leukemia (AMKL) deregulated genes by RNAseq
Acute megakaryoblastic leukemia (AMKL) is a heterogeneous disease generally associated with poor prognosis. Gene expression profiles indicate the existence of distinct molecular subgroups, and several genetic alterations have been characterized in the past years, including the t(1;22)(p13;q13) and t...
ORGANISM(S): Homo sapiens 
2016-05-21 | E-MTAB-4697 | biostudies-arrayexpress
GenomicsIdentification of ETO2-GLIS2 targets by ChIP-sequencing
Acute megakaryoblastic leukemia (AMKL) is a subtype of leukemia primarily diagnosed in childhood and generally associated with poor prognosis. Genetic alterations found in de novo childhood AMKL include the OTT-MAL fusion, MLL and NUP98 fusions and the recently identified ETO2-GLIS2 fusion that invo...
ORGANISM(S): Homo sapiens 
2017-05-22 | E-MTAB-4367 | biostudies-arrayexpress
TranscriptomicsIPSC model of ETO2-GLIS2 expression
To characterize the molecular consequences of ETO2-GLIS2 expression in iPSC-derived cells, we performed a transcriptome analysis on control and two ETO2-GLIS2 clones at day18 of differentiation. From the control, the CD41+CD42- and CD41+CD42+ populations were sorted to obtain normal immature progeni...
ORGANISM(S): Homo sapiens 
2020-04-27 | E-MTAB-7967 | biostudies-arrayexpress
ProteomicsIdentification of regulators of polyploidization presents therapeutic targetsfor treatment of AMKL
Wen Q, Goldenson B, Silver SJ, Schenone M, Dancik V, Huang Z, Wang LZ, Lewis TA, An WF, Li X, Bray MA, Thiollier C, Diebold L, Gilles L, Vokes MS, Moore CB, Bliss-Moreau M, Verplank L, Tolliday NJ, Mishra R, Vemula S, Shi J, Wei L, Kapur R, Lopez CK, Gerby B, Ballerini P, Pflumio F, Gilliland DG, Go...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2014-01-27 | MSV000078536 | MassIVE
TranscriptomicsOntogenic changes in hematopoietic hierarchy determine pediatric specificity and disease phenotype in fusion oncogene-driven myeloid leukemia - scRNAseq - 2
Paediatric cancers present specific genetic alterations, however the principles underlying their differences to adult cancers are yet unclear. Poor prognosis paediatric myeloid leukaemia fusion oncogenes represent clinically-relevant models to investigate age-specific tumorigenesis. Here, we establi...
ORGANISM(S): Mus musculus 
2019-10-15 | E-MTAB-8360 | biostudies-arrayexpress
TranscriptomicsPrimary human CD34+ cells for modeling ETO2-GLIS2 leukemogenesis - Bulk RNAseq
Several fusion oncogenes showing a higher incidence in pediatric acute myeloid leukemia are associated with heterogeneous megakaryoblastic and other myeloid features. Here we addressed how developmental mechanisms influence human leukemogenesis by ETO2::GLIS2, a hallmark of dismal prognosis. We ind...
ORGANISM(S): Homo sapiens 
2024-10-08 | E-MTAB-14131 | biostudies-arrayexpress
TranscriptomicsPrimary human CD34+ cells for modeling ETO2-GLIS2 leukemogenesis - ATACseq
To understand the early consequences of cytokine stimulation, we performed chromatin accessibility assay (ATAC-seq) on primary human CD34+ fetal and cord blood cells exposed or not to IL3 and SCF for 5 days in vitro.
ORGANISM(S): Homo sapiens 
2024-10-08 | E-MTAB-14124 | biostudies-arrayexpress
TranscriptomicsPrimary human CD34+ cells for modeling ETO2-GLIS2 leukemogenesis - Single cell RNAseq
To characterize the consequences of cytokine stimulation, we compared ET02-GLIS2 expressing cells from different origins by single cell transcriptomes (scRNAseq). We analysed FL and CB ET02-GLIS2-expressing CD34+ progeny after 7 days in vitro, human cells from diseased NSG (FL- and FBM-derived) and ...
ORGANISM(S): Homo sapiens 
2024-10-08 | E-MTAB-14140 | biostudies-arrayexpress
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