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GenomicsAmplicon-Screening of genomic DNA regions of mutants in the Exon5 R175 position of TP53 in HCT116 cells generated via CRISPR editing
To compare the impact of CRISPR-egineered R175 TP53 mutant variants in HCT116 and H460 cells, mutations at the amino acid position 175 were generated systematically by CRISP/Cas9 editing. Here, genomic amplicon regions covering the TP53 Exons 5 were sequenced via targeted sequencing.
ORGANISM(S): Homo sapiens 
2024-10-16 | E-MTAB-14322 | biostudies-arrayexpress
TranscriptomicsChipSeq of mouse embryonic fibroblasts from conditional knock-in mouse carrying a phosphorylation-deficient S180A (SA) mutation in exon 5 of the endogenous mouse Trp53 gene locus
The mouse S180A (SA) mutation of the Trp53 is a p53 phosphorylation-deficient mutant protein with native conformation. Here we want to assess the impact of DNA binding site phosphorylation of the p53 target gene spectrum and transcriptional activity under untreated conditions and following p53 stabi...
ORGANISM(S): Mus musculus 
2020-06-04 | E-MTAB-9170 | biostudies-arrayexpress
TranscriptomicsAmplicon sequencing of the trp53 locus of DNA extracted from FFPE sections of tumors obtained from the gastrointestinal tract of APC mice
This experiment was done to analyze the p53 status in gastrointestinal tumors in the colon and small intestine in APC mice carrying either a wild type p53 or a mutant p53 (R172H) allele and compare colon and small intestine tumors.
ORGANISM(S): Mus musculus 
2020-02-25 | E-MTAB-8355 | biostudies-arrayexpress
TranscriptomicsExpression profiling of Eµ-Myc-driven lymphomas using a reversible switchable p53 allele modeling early and late stage p53 inactivation.
For the most frequently inactivated tumor suppressor p53, genetic mouse models have demonstrated regression of p53-null tumors upon p53 reactivation. While this was shown in tumor models driven by p53 loss as the initiating lesion, many tumors initially develop in the presence of wild-type p53, acqu...
ORGANISM(S): Mus musculus 
2019-10-18 | E-MTAB-8015 | biostudies-arrayexpress
GenomicsChipSeq of conditional knock-in mouse carrying the R178E (EE) mutation in exon 5 of the endogenous mouse Trp53 gene locus.
The mouse R178E (EE) mutation of the Trp53 is a p53 mutant protein with native conformation that lacks the ability to form tetramers and thus constitutes a mutant form of p53 that lacks DNA binding cooperativity. Here we want to assess DNA binding ability of the EE mutant in MEFs under untreated con...
ORGANISM(S): Mus musculus 
2019-09-03 | E-MTAB-6793 | biostudies-arrayexpress
TranscriptomicsRNAseq of the protease transcriptome of primary murine alveolar epithelial cells type II (AECII) and the murine lung cells MLE-15
RNAseq of primary murine alveolar epithelial cells type II (AECII) and the murine lung cells MLE-15 in order to identify potential candidates of trypsin-like proteases capable to cleave the influenza surface glycoprotein hemaglutinine (HA). AECII cells do support the cleavage of HA while MLE-15 cell...
ORGANISM(S): Mus musculus 
2020-03-27 | E-MTAB-8571 | biostudies-arrayexpress
TranscriptomicsRNAseq of trachea, bronchi and lungs of mice to investigate expression and distribution of trypsin-like proteases in the murine lower airways.
RNAseq of trachea, bronchi and lungs of mice to investigate expression and distribution of trypsin-like proteases in the murine lower airways in order to identfy potential host cell proteases capable of cleaving influenza surface glycoprotein hemaglutinine (HA) during infection.
ORGANISM(S): Mus musculus 
2020-03-27 | E-MTAB-8573 | biostudies-arrayexpress
GenomicsChIPseq of enterocytes of the jejunum from transgene C57BL/6 mice harboring a CKIαΔgut knock-out
ChIPseq of enterocytes of the jejunum from transgene C57BL/6 mice to assess the effects of a CKIαΔgut knock-out in the jejunum and a CKIαΔgut/p53 double knock-out. In addition, enterocytes expressing a mutant p53 (R172H) in combination with CKIαΔgut knockout were analyzed.
ORGANISM(S): Mus musculus 
2020-02-25 | E-MTAB-7858 | biostudies-arrayexpress
TranscriptomicsOncogenic RAS primes primary acute myeloid leukemia blasts for differentiation
RAS mutations are frequently found among AML patients, generating a constitutively active signaling protein changing cellular proliferation, differentiation and apoptosis. We previously showed that treatment of AML patients with high-dose cytarabine (HDAC) is preferentially beneficial for those with...
2015-11-20 | E-MTAB-2090 | biostudies-arrayexpress
TranscriptomicsRNAseq of HeLa and U2OS cells treated with either Nutlin, Darpin C10 targeting p53 or a control DARPin
p53 expression and transcriptional activity is repressed in human papillomavirus positive cells by the expression of viral E6 protein. E6 forms a complex with cellular E6AP protein which can lead to ubiquitination of p53, thus repressing its tumor-suppressive capabilities. In this experiment, the po...
ORGANISM(S): Homo sapiens 
2025-09-05 | E-MTAB-13602 | biostudies-arrayexpress
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