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ProteomicsIntegrated MICROFASP method with CZE based fractionation technique and nanoRPLC-ESI-MS/MS for comprehensive proteomics analysis of submicrogram sample
We report a loss-less two dimensional (2D) separation platform that integrated capillary zone electrophoresis (CZE) fractionation and nanoRPLC-ESI-MS/MS for comprehensive proteomics analysis of submicrogram sample. Protein digest was injected into the linear polyacrylamide coated capillary followed ...
ORGANISM(S): Homo sapiens (Human) 
2024-08-10 | PXD052735 | Pride
Submicrogram sample Two dimensional separation Capillary zone electrophoresis Microfasp method Proteome profiling 
ProteomicsA fast, high-sensitivity 96-well plate based MICROFASP method for processing low microgram proteomics sample within 1.5 hours
We report a fast, high-sensitivity MICROFASP method that capable of completing sample preparation in less than 1.5 h which improved the throughput over 13 times compared to the previous reports. In this method, protein digestion time was dramatically shortened from 17 h to 20 min in the limited reac...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-05-07 | PXD053720 | Pride
Fast sample preparation  proteomics analysis  high-sensitivity  high-throughput 
ProteomicsAn Optimized Miniaturized Filter Aided Sample Preparation Method for Sensitive Cross-linking Mass Spectrometry Analysis of Microscale Samples
Cross-linking mass spectrometry (XL-MS) is a powerful tool for elucidating protein structures and protein-protein interactions (PPIs) at the global scale. However, sensitive XL-MS analysis of mass-limited samples remains challenging, due to serious sample loss during sample preparation of the low-ab...
ORGANISM(S): Homo sapiens (Human) 
2025-01-03 | PXD051322 | Pride
Cross-linking mass spectrometry Microscale samples 
UnknownSeamlessly Integrated Miniaturized Filter-Aided Sample Preparation Method to Fractionation Techniques for Fast, Loss-Less, and In-Depth Proteomics Analysis of 1 μg of Cell Lysates at Low Cost.
Not available
S-EPMC9897233 | biostudies-literature
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