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To study chondrogenesis, we used a chicken limb bud model: We used RNA sequencing, and examined the differences between gene expression patterns during cartilage formation in micromass cultures of embryonic limb bud-derived progenitors. We sequenced in triplicate at Day 0,1,2,3,4,6,10,15 and also fr...
ORGANISM(S): Gallus gallus 
Primary micromass cultures derived from 11.5 day old mouse embryo limb buds were cultured for 15 days in differentiating conditions (beta-glycerophosphate and ascorbic acid). Total RNA from differentiating chondrocytes was isolated every three days i.e. days 3,6,9,12 and 15 and hybridized to MOE430A...
ORGANISM(S): Mus musculus 
2005-09-08 | GSE2154 | GEO
Micromass Time Course
Bovine articular chondrocytes were grown in micromass culture and were either untreated or treated with 5 ng TGF-b1/ml for 8 hours to identify genes regulated by TGF-b. Six total samples were analyzed. Three biological replicates of untreated bovine articular chondrocytes grown in micromass culture ...
ORGANISM(S): Bos taurus 
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