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TranscriptomicsPromoter capture Hi-C of CD34+ haematopoietic stem cells
CD34+ heamatopoietic stem cells were isolated from the bone marrow of two healthy donors undergoing total hip replacement. Promoter capture Hi-C (PCHi-C) was performed on these cells using the protocol according to Mifsud et al. 2015, with the exception that ligation was performed in situ, and a sl...
ORGANISM(S): Homo sapiens 
2021-10-15 | E-MTAB-10701 | biostudies-arrayexpress
GenomicsPromoter capture mESC and fetal liver
Genome organization influences transcriptional regulation by facilitating interactions between gene promoters and distal regulatory elements. To analyse distal promoter contacts we used Capture Hi-C (CHi-C) to enrich for promoter-interactions in HiC libraries from mouse ESC and E14.5 fetal liver. Pl...
ORGANISM(S): Mus musculus 
2015-02-01 | E-MTAB-2414 | biostudies-arrayexpress
TranscriptomicsPromoter Capture Hi-C of mouse ESC and TSC
Chromatin organisation of trophoblast stem cells (TSC) were compared with that of embryonic stem cells (ESC). The method enriches Hi-C libraries, to detect promoter interactions at restriction fragment level. We prepared Hi-C libraries from TSC and ESC (serum grown) samples and enriched them with a ...
ORGANISM(S): Mus musculus 
2018-09-05 | E-MTAB-6585 | biostudies-arrayexpress
GenomicsPromoter Capture-HiC in Ring1a KO and Ring1a/b dKO mouse ES cells
Genome organization influences transcriptional regulation by facilitating interactions between gene promoters and distal regulatory elements. To analyse distal promoter contacts mediated by the PRC1 complex we used Capture Hi-C (CHi-C) to enrich for promoter-interactions in a HiC library in Ring1a K...
ORGANISM(S): Mus musculus 
2015-08-01 | E-MTAB-3109 | biostudies-arrayexpress
GenomicsH3K4me1 and H3K27ac ChIP-seq in Ring1a KO and Ring1a/b dKO mouse ES cells
H3K4me1 (ab8895 Abcam) and H3K27ac (ab4729 Abcam) antibodies were used for ChIP-seq in Ring1a-/- mouse ES cells and after 48h tamoxifen treatment in conditional knock-out of Ring1b in the Ring1a -/- background.
ORGANISM(S): Mus musculus 
2015-08-01 | E-MTAB-3156 | biostudies-arrayexpress
TranscriptomicsNuclear RNA-seq in WT, Ring1a KO and Ring1a/b dKO mouse ES cells
Nuclear RNA was isolated from all three cell types to enable differential expression analysis of both coding and non-coding RNA shortly after tamoxifen treatment, that resulted in conditional knock-out of Ring1b in the Ring1a -/- background.
ORGANISM(S): Mus musculus 
2015-08-01 | E-MTAB-3125 | biostudies-arrayexpress
GenomicsHi-C in mouse fetal liver cells
HindIII Hi-C dataset from mouse fetal liver cell samples to assess the performance of interaction calling algorithms.
ORGANISM(S): Mus musculus 
2016-02-01 | E-MTAB-3891 | biostudies-arrayexpress
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