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ProteomicsBONCAT in Arabidopsis cell culture LC-MS
Determining which proteins are actively synthesised at a given point in time and being able to extract them for an...
...ino acid sequence of proteins and correlation with independent studies of protein labelling with 15N verified the data. We provide evidence that HPG-based BONCAT tags nascent plant proteins in a superior manner to azidohomoalinine (AHA)-based BONCAT in Arabidopsis and show that AHA’s induction of Met metabolism and greater inhibition of cell growth rate than HPG likely limits AHA incorporation at Met sites in Arabidopsis. HPG-based BONCAT provides a verifiable method for determining which plant proteins are being synthesised at a given time point and enriches new protein molecules from the bulk protein pool for identification, quantitation and subsequent biochemical analysis.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-06-23 | PXD023170 | Pride
Boncat Arabidopsis Lc-ms Cell culture Noncanoical amino acids 
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ProteomicsSgt1 interactome analysis under proteostatic stress and steady state conditions
Buildup of misfolded proteins are prevented by chaperone-assisted refolding and/or proteasome dependent degradation. The coordination of these two protein quality control (PQC) systems is not fully understood. We identified the essential Hsp90 co-chaperone Sgt1 as a new member of a general PQC linking...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-07-02 | PXD016174 | Pride
Heat shock Sgt1 Hapi Saccharomyces cerevisiae Protein quality control 
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ProteomicsMapping the Triticum aestivum proteome
Wheat is the staple food of over 35% of the world’s population, accounts for 20% of all human calories, and it...
...nt. The specific functions of wheat organs during vegetative development and the role of metabolism, protein degradation and remobilisation in driving grain production are the foundations of crop performance and have recently become accessible through studies of the wheat proteome. With the aim of creating a resource complementary to current genome sequencin...
ORGANISM(S): Triticum aestivum (Wheat) 
2016-11-17 | PXD004720 | Pride
Proteomics Lcms Proteome Database Triticum aestivum Spectral counting Wheat organs Mapping Wheat 
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ProteomicsSND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Regulation of viral RNA biogenesis is fundamental to productive SARS-CoV-2 infection. To characterize host RNA-binding proteins (RBPs) involved in this process, we biochemically identified proteins bound to genomic and subgenomic SARS-CoV-2 RNAs. We find that the host protein SND1 binds the 5ʹ end of...
ORGANISM(S): Homo sapiens (Human) 
2023-10-05 | PXD037528 | Pride
Sars-cov-2 Rna-binding proteins Rna interactome 
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ModelsGould2013 - Temperature Sensitive Circadian Clock
Gould2011 - Temperature Sensitive Circadian Clock This model is a temperature sensitive version of Pokhil...
...ambient temperatures. Gould PD, Ugarte N, Domijan M, Costa M, Foreman J, Macgregor D, Rose K, Griffiths J, Millar AJ, Finkenstädt B, Penfield S, Rand DA, Halliday KJ, Hall AJ. Mol. Syst. Biol. 2013; 9: 650 Abstract: Circadian clocks exhibit 'temperature compensation', meaning that they show only small cha...
2024-09-02 | BIOMD0000000564 | BioModels
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TranscriptomicsTranscription profiling of Arabidopsis suspension cells exposed to a range of abiotic treatments
Profiling the transcriptome of Arabidopsis suspension cells in response to a range of abiotic treatments. Experiment Overall Design: variable_1 = 3 h Experiment Overall Design: variable_2 = 12 h Experiment Overall Design: variable_3 = untreated, control Experiment Overall Design: variable_4 = hydroge...
ORGANISM(S): Arabidopsis thaliana 
2007-07-11 | E-GEOD-3709 | biostudies-arrayexpress
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UnknownA common mechanism by which type 2A von Willebrand disease mutations enhance ADAMTS13 proteolysis revealed with a von Willebrand factor A2 domain FRET construct.
Not available
S-EPMC5706690 | biostudies-literature
0
UnknownFRET Characterization of Complex Conformational Changes in a Large 16S Ribosomal RNA Fragment Site-Specifically Labeled Using Unnatural Base Pairs.
Not available
S-EPMC4874843 | biostudies-literature
0
UnknownRevisiting pneumococcal carriage by use of broth enrichment and PCR techniques for enhanced detection of carriage and serotypes.
Not available
S-EPMC2863911 | biostudies-literature
0
UnknownAssessment of intra-fraction motion during frameless image guided Gamma Knife stereotactic radiosurgery.
Not available
S-EPMC9883231 | biostudies-literature
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