WT or DJ-1 KO HeLa cells (n=3) were lysed in 500 μL of 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 x g for 15 min at 4 ºC. The supernatants were recovered and proteins (1 mg each) ...
ORGANISM(S): Homo Sapiens (human)
