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TranscriptomicsHCT 116 cells expressing Fucci (fluorescent ubiquitination-based cell cycle indicator); G1 phase vs. S/G2/M phase
Fucci-expressing HCT116 were inoculated into subcutaneous tissue or cecum of NOD/SCID mice. Four weeks after inoculation, Fucci green (mAG; S/G2/M) and red (mKO2;G1) expressing cells were isolated from implanted tumors by FACSAria sorting (BD, Biosciences). Comparative analyses among green and red c...
ORGANISM(S): Homo sapiens 
2014-01-09 | E-GEOD-34940 | biostudies-arrayexpress
TranscriptomicsExpression data of NIH3T3 in G0 and G1 phases
NIH3T3 in the middle of G0 to G1 transion consists of the cells which is still staying G0 phase and the cells which enters G1. Monitoring the expressions of p27 and Cdt1 enables to distinguish these two; p27+/Cdt1+ cells as the cells in G0 phase and p27-Cdt1+ cells as G1 phase We sorted p27+Cdt1+ (...
ORGANISM(S): Mus musculus 
2014-02-21 | E-GEOD-46511 | biostudies-arrayexpress
TranscriptomicsGene expression profile of influenza virus antigen-activated or in vitro stimulated murine CD8+ T cells on day 7
The precise timing and pathway of memory CD8+ T cells differentiation from naïve T cells have remained undetermined. We found the smaller cell-size and slower cell cycling cells were segregated from the proliferative larger cell-size activated T cell pool at the peak of infection. Gene signature of ...
ORGANISM(S): Mus musculus 
2015-02-25 | E-GEOD-48219 | biostudies-arrayexpress
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