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To characterize Klf4 downstream genes, we performed microarray analyses of the ES cells knocked down by siRNA of Klf4 gene. Keywords: individual genetic characteristic design ES cells grow without feeders on gelatin coated in complete ES medium. Cells were incubated at 37 0C; 5% CO2. ES cells were t...
ORGANISM(S): Mus musculus 
Cell culture and establishment of cell lines The mouse pancreatic beta cell line MIN6 at passage 24 was obtained with permission from Dr. Jun-ichi Miyazaki at Osaka University (Miyazaki et al., 1990). MIN6 cells were cultured as described previously (Miyazaki et al., 1990). Two independent Sirt1-ove...
ORGANISM(S): Mus musculus 
2005-04-02 | GSE2365 | GEO
mSirt1 overexpression and knockdown in a pancreatic beta-cell line
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